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AB0410 The Influence of Magnetic Properties of The Adsorbents on Efficiency of Extracorporeal Therapy in Systemic Lupus Erythematosus with Antiphospholipid Syndrome
  1. I.P. Gontar1,
  2. O.I. Emelyanova1,
  3. L.A. Maslakova1,
  4. O.V. Paramonova1,
  5. I.A. Zborovskaya2
  1. 1Clinical Immunology Lab
  2. 2FSBSU Research Institute for Clinical and Experimental Rheumatology, Volgograd, Russian Federation

Abstract

Background Due to magnetic properties an installation which creates physiological magnetic field holding granules in suspension to prevent particle adhesion of sorbent and contribute to better blood contact was used.

Objectives To remove pathogenic antibodies to cardiolipin in patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS), constant hemocompatible antigenic drugs with magnetic properties (immobilized granulated antigen drugs – IGAD) were created.

Methods To determine antibodies level to cardiolipin (AKA) the indirect ELISA was used. Magnetic adsorbents were created using technique of 3D oriented antigen fixation.

Results When carrying out sorption of antibodies to cardiolipin (CL) in vitro, its decrease in all cases of EGAD use was observed (before sorption of antibodies to CL – 0.390±0.021, after – 0.141±0,006, p≤0.001) unlike hemosorption with activated coal (before coal hemoadsorbent (HSC) – 0.52±0.05, after – 0.44±0.04, p>0.05). The use of activated carbon was accompanied by significant damage of formed blood elements: erythrocytes (1012 /l) 3.8±0.02 – prior to sorption, 3.3±0.02 – after sorption; leukocyte count (109 /l) 5.4±0.12 before sorption, 4.0±0.09 after sorption; platelets (109/l) 203±1.14 – prior to sorption, 146±0.97 – after sorption that wasn't observed when IGAD used (erythrocytes (1012 /l) – prior to sorption 3.8±0.03; after sorption 3.8±0.03; leukocytes (109 /l) – prior to sorption 5.4±0.12; after – 5.3±0.14; platelets (109/l) – prior to sorption 203±1.17; after 200±1.53.

Thrombosis frequency in the column free of magnetic field made 8 cases out of 20, and in 4 of them prevented blood flow. When carrying out immunoadsorbtion procedure in installation mentioned, 1 case out of 20 microclots were detected, which were not an obstacle to successful completion of immunoadsorbtion (χ2 =7.03, p=0.009). As a result of sorption procedure a significant decrease in circulated immune complexes (CIC) concentration from 12.4±1.2 to 4.6±0.5% was observed, at the same time changes in the concentration of total protein, immunoglobulin G, A, M classes were insignificant (p<0.05).

Conclusions Thus, the adsorbent magnetic properties allow to avoid formed blood elements destruction as well as to increase extracorporal immunoadsorbtion efficiency, especially in cytopenic syndrome often observed in patients with SLE with APS.

  1. I. P. Gontar, O. I. Yemelyanova, L. A. Maslakova, N. M. Zolotareva, N. And. Yemelyanov, A. N. Krasilnikov, I. A. Zborovskaya. Immunoadsorbtion therapy as a factor pathological agents biocorrection in patients with systemic lupus erythematosus with antiphospholipid syndrome. 4th International Interdisciplinary Conference on “modern problems in systemic regulation of physiological functions”. Conference Proceedings. Moscow, Russia, September 17–18, 2015. P 157–160.

Disclosure of Interest None declared

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