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Pitfalls for detecting interleukin-33 by ELISA in the serum of patients with primary Sjögren syndrome: comparison of different kits
  1. Elodie Rivière1,2,
  2. Bineta Ly2,
  3. Saida Boudaoud2,
  4. Houria Chavez2,
  5. Gaetane Nocturne1,2,
  6. Philippe Chanson3,
  7. Corinne Miceli Richard1,2,
  8. Xavier Mariette1,2
  1. 1 Rheumatology Department, Université Paris-Sud, Assistance Publique-Hôpitaux de Paris, Le Kremlin-Bicêtre, France
  2. 2 INSERM U1184, Université Paris-Sud, Le Kremlin-Bicêtre, France
  3. 3 Endocrinology Department, Université Paris-Sud, Assistance Publique-Hôpitaux de Paris, Le Kremlin-Bicêtre, France
  1. Correspondence to Professor Xavier Mariette, Hôpitaux Universitaires Paris-Sud, Université Paris-Sud, 78 rue du Général Leclerc, Le Kremlin Bicêtre 94270, France; xavier.mariette{at}aphp.fr

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The recent interest for interleukin-33 (IL-33) in primary Sjögren syndrome (pSS) pathophysiology is due to three studies,1–3 which have shown an increase in IL-33 serum levels in patients with pSS compared with controls. IL-33 is a member of the IL-1 family which is released in case of cellular damage and acts as an alarmin; however, its role in pSS physiopathology remains unclear.

In the present study, we aimed to replicate the association described between IL-33 serum level and pSS diagnosis. For that purpose, we used Quantikine and Duoset ELISA (R&D Systems, Minnesota, USA) and Luminex (Milliplex HTh17, Merck, Darmstadt, Germany). The Quantikine and Luminex kits are validated for serum samples, while the Duoset is only validated for use with culture supernates (data from R&D). IL-33 serum levels were assessed in patients with pSS from the French multicentre prospective ASSESS cohort (Assessment of Systemic Signs and Evolution in Sjögren Syndrome, principal investigator: X …

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