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AB0192 High Expression of MIR-16 in PBMC of SLE Patients
  1. Z. Ye,
  2. Z. Yin,
  3. H. Zeng,
  4. W. He
  1. Rheumatology Institute of Guangdong Medical College, Shenzhen, China

Abstract

Background Systemic lupus erythematosus (SLE) is a predominantly female complex autoimmune disease characterized by the production of autoantibodies against a host of nuclear antigens and immune complex deposition in organs. Recent studies have revealed the potential contribution of miRNAs in the regulation of signaling pathways and autoimmune genes in lupus, providing insights into the pathogenesis of SLE.

Objectives To determine the expression of miR-16 in PBMC from Systemic lupus erythematosus (SLE) patients

Methods Peripheral blood mononuclear cells (PBMC) were separated from peripheral blood of 16 SLE patients and 12 healthy individuals. Total RNAs were isolated and purified. The level of miR-16 was determined by quantitative reverse transcription PCR (qRT-PCR). U6 was used as housekeeping control. The amount of target miRNA was normalized relative to the amount of U6 (ΔCt =ΔCtmiRNA-ΔCtU6). Relative expression levels were expressed as 2-Δ Ct.

Results The expression level of miR-16 was significantly higher in the SLE patients than that in the healthy control group (919.87±715.45 vs 413.6 3±330.69, P=0.02). And it was also significantly higher in SLE active group than that in SLE stable group (540.95±350.15 vs 1298.79±803.79, t=-2.445, P<0.05). The level of miR-16 was related with AnuA (r=0.669, P<0.01),ESR (r=0.608, P<0.05) and SLEDAI (r=0.530, P<0.05). The level of miR-16 was not related with anti-dsDNA antibody, CRP, C3 and C4.

Conclusions The expression of miR-16 is high in SLE patients and it is related with SLE activity.

It may be a potential non-invasive biomarker for SLE diagnosis and SLE activity assessment.

References

  1. Wang H, Peng W, Ouyang X, et al. Circulating microRNAs as candidate biomarkers in patients with systemic lupus erythematosus [J]. Transl Res, 2012,160(3):198-206.

  2. Pauley KM, Satoh M, Chan AL, et al. Upregulated miR-146a expression in peripheral blood mononuclear cells from rheumatoid arthritis patients [J]. Arthritis Res Ther, 2008, 10(4):R101.

  3. Murata K, Yoshitomi H, Tanida S, et al. Plasma and synovial fluid microRNAs as potential biomarkers of rheumatoid arthritis and osteoarthritis [J]. Arthritis Res Ther, 2010;12(3):R86.

  4. Pekarsky Y, Croce CM. Role of miR-15/16 in CLL. Cell Death Differ. 2015,22(1):6-11.

Disclosure of Interest None declared

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