Background Rheumatoid arthritis (RA) is one of the most common rheumatic diseases, and Its main pathological manifestation is a highly vascularized tissue caused by infiltration of T, B cells and macrophages. CyPA could regulate the expression level of CD147 on cell membrane. which to promote leukocytes of inflammatory response to infiltrate the lesion tissues and played an important role in RA. It suggests that CyPA may induce the chemical chemotaxis by CD147 of neutrophils and monocytes, and induce the chemotaxis of inflammatory cells into synovial fluid of joint cavity, which leads to articular cartilage and bone destruction of RA patients and eventually promotes the development of the disease. However, the underlying inflammatory response of acquired immune response and the interaction between cyclophilin A and CD147 at the B-cell activation stage when specific antibodies are produced still remains unclear.
Objectives To study the effect of CypA on CD147 expression on B cells in synovium and synovial fluid, and its effect on autoantibodies secreted by B cells.
Methods Immunohistochemical staining (B cell, follicular dendritic cell, and plasma cell were labeled by CD19, CD35, and CD38) was used to observe the formation of germinal center of B cell in RA synovial tissues. Mononuclear cells in RA synovial fluid were separated and treated with CypA, BAFF and LPS to detect the changes of CD147 expression (the percentage of positive cells and mean fluorescence intensity were recorded) on CD19+ B cells surface by flow cytometry. Supernatant was collected to detect RF subtypes and the level of anti-CCP antibody.
Results There were special structures like germinal centers in synovial specimens in 13 out of 35 RA patients. The expression of CD147 at different degrees was noted. In CD147-positive cells in RA synovial lymphatic follicle, 30±8% was lymphocyte, 32±7% was follicular dendritic cells, and 38±11% was endothelial cells. Weak expression or no expression of plasma cell was also recorded. The highest CD147 MFI of CD19+ B appeared 48 h after CypA, BAFF and LPS treatment. After treatment, RF and anti-CCP of supernatant were significantly higher than those before treatment (p<0.01). There was no significant difference among CypA, BAFF and LPS (p>0.05).
Conclusions The interaction between CypA and CD147 involves in the pathogenesis of B cell activation and inflammatory response in RA. Further study will provide new clues for targeted anti-inflammatory therapies.
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Disclosure of Interest None declared
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