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AB0125 Differential Expression of Vascular Inflammation Markers in Collagen-Induced Arthritis and Experimental Atherosclerosis
  1. A. Denys1,2,
  2. G. Clavel1,3,
  3. D. Lemeiter1,2,
  4. O. Schischmanoff4,
  5. E. Assier1,2,
  6. M.-C. Boissier2,5,6,
  7. L. Semerano1,2,6
  1. 1Inserm UMR 1125
  2. 2Sorbonne Paris Cité - Université Paris 13, Bobigny
  3. 3Department of Internal Medicine, Fondation Rothschild, Paris
  4. 4Inserm UMR 978
  5. 5Rheumatology, Inserm UMR 1125
  6. 6Department of Rheumatology, Assistance Publique – Hôpitaux de Paris (AP-HP) Groupe hospitalier Avicenne - Jean Verdier – René Muret, Bobigny, France

Abstract

Background Cardiovascular disease (CVD) is a major cause of morbidity and mortality in rheumatoid arthritis (RA). Increased cardiovascular risk in RA is attributed to both traditional risk factors and systemic inflammation over a long period. Limited experimental data exist on vascular involvement in arthritis models1,2,3. Thus, we developed a model that would mimic both vascular dysfunction and articular inflammation in RA, using collagen-induced arthritis (CIA) in in C57Bl/6 (B6) mice.

Objectives To study the expression of vascular inflammatory markers and atheroma formation in CIA with and without concomitant hyperlipidic diet (HD).

Methods CIA was induced in B6 mice with chicken type-II collagen (cCII) followed by a boost 21 days later. These mice were fed with a standard (chow) diet or with HD given for 12 weeks starting from the day of the boost. The control mice were not immunized (NI) but were fed either the standard diet or HD. Arthritis severity was evaluated using a validated clinical score. Aorta and synovial membranes were removed 15 weeks after the first cCII immunization. We analysed vascular cell adhesion molecule-1 (VCAM-1), inducible NO-synthase (iNOS) and interleukin-17 mRNA level in aorta with real-time quantitative PCR. VCAM-1 localisation in the aortic sinus was determined by immunohistochemistry. In addition, atherosclerotic plaques were assessed in the aorta.

Results CIA was associated with high expression of VCAM-1, independently of the fed diet. VCAM-1 overexpression was detectable 4 weeks after cCII immunization and persisted at 15 weeks. HD induced atheroma plaque formation and aortic iNOS expression which were both independent from CIA. Concomitant CIA and HD had no additive effect on atheroma, VCAM-1 or iNOS expression.

Conclusions In B6 mice, HD and CIA induce distinct and independent expression of large vessel inflammation markers. This model, sensitive to both CIA and experimental atherosclerosis may be relevant for the study of CVD in RA.

References

  1. Asquith, D. L., Miller A. M., Hueber A. J., et al. Apolipoprotein E-deficient mice are resistant to the development of collagen-induced arthritis. Arthritis Rheum 2010.62:472-7.

  2. Postigo, J., Genre F., Iglesias M., et al. Exacerbation of type II collagen-induced arthritis in apolipoprotein E-deficient mice in association with the expansion of Th1 and Th17 cells. Arthritis Rheum 2011.63:971-80.

  3. Rose, S., Eren M., Murphy S., et al. A novel mouse model that develops spontaneous arthritis and is predisposed towards atherosclerosis. Ann Rheum Dis 2013.72:89-95.

Disclosure of Interest None declared

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