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AB0032 In-Depth Characterization of Cd24Highcd38High Transitional Human B Cells Reveals Different Regulatory Profiles and Are Abnormally Distributed in Autoimmune Diseases
  1. Q. Simon1,
  2. J.-O. Pers1,
  3. D. Cornec1,2,
  4. M.A. Rizgar3,
  5. S. Hillion1
  1. 1CHRU Morvan, INSERM ESPRI 29, Immunology and Immunotherapy, Brest cedex
  2. 2Rheumatology Department, CHRU Morvan, Brest, France
  3. 3Queen Mary University of London, William Harvey Research Institute, London, United Kingdom

Abstract

Background CD24high CD38high transitional B cells represent a key stage in the developmental pathway of B cell peripheral tolerance and functional maturation. These B cells have been widely ascribed regulatory functions. However, the phenotypic and functional overlap between these cells and regulatory B cells remains controversial.

Objectives In this study, we use multi-color flow cytometry with bioinformatic analyses and functional studies to show that CD24high CD38high B cells can be differentiated into multiple subsets.

Results The study also reveals for the first time that human transitional B cells encompass transitional type 1 (T1) and T2 B cells but also distinct anergic T3 B cells as well as IL-10-producing CD27+ transitional B cells. Interestingly, the latter two subsets differentially regulate CD4+ T cell proliferation and polarization towards Th1 effector cells. Additional analyses show that the percentage of T3 B cells is reduced while the frequency of CD27+ transitional B cells is increased in patients with autoimmune diseases compared with matched healthy individuals.

Conclusions This study provides evidence for the existence of different transitional B cell subsets each displaying unique phenotypic and regulatory functional profiles.Furthermore, the study indicates that altered distribution of transitional B cells subsets highlights different regulatory defects in autoimmune diseases.

Disclosure of Interest None declared

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