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AB0028 The Abnormal CD4+T Lymphocyte Subset Distribution Shown by Naïve Rheumatoid Arthritis Patients Can be Modulated by Methotrexate Treatment
  1. C. Bohόrquez1,2,
  2. L. Ruiz1,2,
  3. C. Garcia2,3,
  4. A. Gόmez-La Hoz2,3,
  5. A. Turriόn1,2,
  6. H. Moruno1,2,
  7. A. Pérez1,2,
  8. A.I. Sánchez1,2,
  9. E. Cuende1,2,
  10. A. Movasat1,2,
  11. F. Albarrán1,2,
  12. M.J. Leόn1,
  13. D. Díaz2,3,
  14. J. Monserrat2,3,
  15. M. Άlvarez-Mon1,2,3
  1. 1Immune System Diseases-Rheumatology Service, University Hospital “Príncipe de Asturias
  2. 2Deparment of Medicicine
  3. 3Laboratory of Inmune System Diseases, University Hospital “Príncipe de Asturias”, University of Alcalá, Alcalá de Henares, Spain

Abstract

Background Mechanisms regulating the chronic autoimmune response in rheumatoid arthritis (RA) are not well understood. However, activated T CD4+ play a pivotal role initiating and perpetuating the chronic inflammation characteristic for the disease.

Objectives Evaluate the number and distribution of circulating CD4+ T lymphocytes and their CD4+ naïve T cells (TN), central memory (TCM), non-terminated effector memory (TNTEM) and terminated effector memory (TTEM) T cells subsets in a population of recently diagnosed DMARD naive RA patients before and along the first 6 months of methotrexate (MTX) treatment.

Methods The number of circulating CD4+ T lymphocytes, and of their TN, TM, TCM and TEM subsets in fifty untreated patients with RA before MTX treatment and at 3 and 6 months of treatment were assayed using a multiparametric flow cytometry. We also studied twenty-four age- and sex-matched healthy subjects as controls.

Results RA naïve patients show a significant (p<0.05) expansion of the circulating CD4+ TNTEM subset. MTX non responder naïve RA patients show from basal conditions a significative expansion of CD4+ TNTEM lymphocytes and develop significant expansion of CD4+ TCM, and CD4+ TEM lymphocyte subsets along MTX treatment.

Conclusions Recently diagnosed DMARD naive RA patients show involvement of the circulating CD4+ T lymphocytes activation/differentiation stage subsets. MTX treatment show immunomodulatory effects on CD4+ T lymphocyte compartment with different behavior in responder and non-responder patients.

Disclosure of Interest None declared

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