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FRI0042 Anti-Carbamylated Protein Antibodies in Unaffected First-Degree Relatives of Rheumatoid Arthritis Patients: Lack of Correlation with Anti-Cyclic Citrullinated Protein Antibodies and Rheumatoid Factor
  1. I.S. Bartosiewicz,
  2. C. Alessandri,
  3. M. Pendolino,
  4. R. Mancini,
  5. T. Colasanti,
  6. A. Pecani,
  7. A. Mastrangelo,
  8. D. Sabatinelli,
  9. V. Riccieri,
  10. F. Conti,
  11. M. Di Franco,
  12. G. Valesini,
  13. F. Morello
  1. University La Sapienza of Rome, Rome, Italy

Abstract

Objectives To investigate the prevalence of anti-carbamylated protein antibodies (anti-CarP) in the Healthy first-degree relatives (HFDRs) of patients with Rheumatoid Arthritis (RA).

Methods We enrolled 141 HFDRs of 63 patients with RA diagnosed accordingly to the 2010 ACR/EULAR criteria. Fifty-six normal healthy subjects (NHS), sex and age matched, served as controls. Anti-CarP IgG, anti-cyclic citrullinated peptide antibody (anti-CCP) IgG and rheumatoid factors (RF) isotypes (IgG, IgA, IgM) were assessed by solid-phase ELISA.

Results Anti-CarP were detectable in 13 HFDRs (9.2%), anti-CCP in 9 (6.3%), IgG-RF in 10 (7%), IgA-RF in 17 (12%), and IgM-RF in 13 (9.2%) HFDRs. Twenty-nine (46%) RA patients were positive for anti-CarP, 31 (49.2%) for anti-CCP, and 34 (53.9%) for RF. One NHS (1.7%) resulted positive for anti-CarP, none for anti-CCP and RF. Anti-CarP showed a significantly higher prevalence in HFDRs than in NHS (p<0.0001). A significant correlation between anti-CCP and RF were found among RA patients (p=0.0002), whereas no correlations were reported between autoantibodies tested in the HFDRs.

Conclusions Anti-CarP can be found in the sera of HFDRs of RA patients and their prevalence is significantly higher than in NHS. No correlation of anti-CarP with anti-CCP and RF antibodies in RA HFDRs was found.

References

  1. Van Veelen PA, et al.: Autoantibodies recognizing carbamylated proteins are present in sera of patients with rheumatoid arthritis and predict joint damage. Proc Natl Acad Sci U S 2011; 42: 17372-7.

  2. Shi J et al.: Anti-carbamylated protein antibodies are present in arthralgia patients and predict the development of rheumatoid arthritis. Arth Rheum 2013; 4: 911-5.

  3. Shi J et al.: Anti-carbamylated protein antibodies are present in arthralgia patients and predict the development of rheumatoid arthritis. Arth Rheum 2013; 4: 911-5.

Acknowledgements This work has been supported by Fondazione Umberto Di Mario ONLUS.

We thank Giuliana Giuliani and Stefania Mieli for their technical support

Disclosure of Interest None declared

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