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FRI0009 Decreased Frequencies of Circulating CD19+CD24highCD38high B Cells in ACPA+ Rheumatoid Arthritis (RA) Patients
  1. I. Arroyo-Villa1,
  2. M.-B. Bautista-Caro1,
  3. A. Balsa1,
  4. P. Aguado1,
  5. M.-G. Bonilla-Hernán1,
  6. C. Plasencia1,
  7. A. Villalba1,
  8. L. Nuño1,
  9. A. Puig-Kröger2,
  10. E. Martín-Mola1,
  11. M.-E. Miranda-Carús1
  1. 1Department of Rheumatology, Hospital La Paz - IdiPaz
  2. 2Department of Immunology, Hospital Gregorio Marañόn, Madrid, Spain

Abstract

Background CD19+CD24highCD38high B cells have been described to have a regulatory capacity and their frequency is altered in the peripheral blood of patients with various autoimmune diseases.

Objectives To determine the frequency of circulating CD19+CD24highCD38high B cells in patients with RA, and establish a correlation with clinical parameters.

Methods Peripheral blood was drawn from RA patients treated with non-biological DMARDs (n=48), RA patients treated with anti-TNFalpha drugs (n=15) and healthy controls (n=63) that were matched with patients for age and gender. After isolation by Ficoll-Hypaque gradient, PBMCs were stained with antibodies to CD3, CD4, CD19, CD24, and CD38, and examined by flow cytometry.

Results A decreased frequency of CD19+CD24highCD38high B cells was apparent in in ACPA+ but not ACPA- RA patients, and this was observed not only in patients treated with non-biological DMARDs but also in patients receiving anti-TNFalpha drugs. In addition, the frequency of CD19+CD24highCD38high B cells showed a significant negative correlation with ACPA titers (r = -0.43, p=0.023). In contrast, no correlation was found between the frequency of circulating CD19+CD24highCD38high B cells and either rheumatoid factor titres or disease activity as determined by the DAS28 score.

Conclusions A decreased frequency of CD19+CD24highCD38high B cells is observed in ACPA+ but not ACPA- RA patients treated with either nonbiological DMARDs or anti-TNFalpha drugs, that is related with ACPA titres but not with rheumatoid factor titres or disease activity.

Disclosure of Interest None declared

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