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THU0093 Altered Expression of CD4+CD28- T Lymphocytes in Methotrexate Treated Rheumatoid Arthritis Patients
  1. C. Bohόrquez1,2,
  2. L. Ruiz1,2,
  3. C. Garcia2,3,
  4. A. Gόmez-La Hoz2,3,
  5. A. Turriόn1,2,
  6. H. Moruno1,2,
  7. A. Pérez1,2,
  8. A.I. Sánchez1,2,
  9. E. Cuende1,2,
  10. A. Movasat1,2,
  11. F. Albarrán1,2,
  12. M.J. Leόn1,
  13. D. Díaz2,3,
  14. J. Monserrat2,3,
  15. M. Άlvarez-Mon1,2,3
  1. 1Immune System Diseases-Rheumatology Service, University Hospital “Príncipe de Asturias”
  2. 2Deparment of Medicicine
  3. 3Laboratory of Inmune System Diseases, University Hospital “Príncipe de Asturias”, University of Alcalá, Alcalá de Henares, Spain

Abstract

Background There are evidences supporting an expansion of CD4+ T lymphocytes in chronic and treated rheumatoid arthritis (RA) patients. The potential increased presence of these T cells in early RA remains elusive.

Objectives Evaluate the number and distribution of circulating CD4+ T lymphocytes and their CD4+ naïve T cells (TN), central memory (TCM), non-terminated effector memory (TNTEM) and terminated effector memory (TTEM) T cells subsets according to the CD28 expression in a population of recently diagnosed DMARD naive RA patients before and along the first 6 months of methotrexate (MTX) treatment.

Methods The number of circulating CD4+ T lymphocytes including their activation/differentiation stages (TN, TM, TCM and TEM subsets) and the CD28 expression, in fifty untreated patients with RA before MTX treatment and at 3 and 6 months of treatment, were assayed using multiparametric flow cytometry. We also studied twenty-four age- and sex-matched healthy subjects as controls.

Results RA naïve patients show a significant (p<0.05) expansion of the circulating CD4+CD28- T lymphocytes. The increased of this subset is mainly due to a significant elevator in the numbers of CD4+CD28- T naïve lymphocytes, CD4+CD28- T central memory lymphocytes and CD4+CD28- T non terminated effector memory lymphocytes.

Conclusions In this work, we have found that the expansion of CD4+CD28- T lymphocytes is already detected in recently and DMARD AR naïve patients. Moreover this increase of CD28- T lymphocytes is also found in CD4+ T naïve lymphocytes. This it is possible to suggest that the expansion of CD4+CD28- occurs associated to the development of the RA as a biological hallmark of the disease.

Disclosure of Interest None declared

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