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THU0065 Immunomodulatory Effects of Dietary Non-Digestible Oligosaccharides in T Cell-Mediated Autoimmune Arthritis
  1. R. Rogier1,
  2. T. Ederveen2,
  3. A. Hartog3,
  4. B. Walgreen1,
  5. L. van den Bersselaar1,
  6. M. Helsen1,
  7. P. Vos3,
  8. J. Garssen3,4,
  9. L. Willemsen4,
  10. W. van den Berg1,
  11. M. Koenders1,
  12. S. Abdollahi-Roodsaz1
  1. 1Experimental Rheumatology
  2. 2Centre for molecular and biomolecular informatics, Radboud university medical center, Nijmegen
  3. 3Danone Nutricia Research, Danone Nutricia Research
  4. 4Division of phamacology, Utrecht university, Utrecht, Netherlands

Abstract

Background Accumulating evidence indicates the relevance of intestinal microbiota in shaping the immune response and supports its contribution to the development of autoimmune diseases. Prebiotic non-digestible oligosaccharides are known to selectively support growth of commensal Bifidobacteria and Lactobacilli and adjust the microbiota composition.

Objectives The aim of this study was to assess the efficacy of microbiota modulation using non-digestible oligosaccharides as a therapeutic approach for T cell-dependent autoimmune arthritis.

Methods IL-1 receptor antagonist (IL-1Ra) deficient mice spontaneously developing an autoimmune T cell-dependent arthritis were used for this study. To examine the feasibility of microbiota modulation as a therapeutic approach during established disease, IL-1Ra-/- mice which had already developed arthritis under conventional microbial status were orally fed a prebiotic diet containing short-chain galacto- and long-chain fructooligosaccharides (scGos:lcFos, 9:1). Disease progression was monitored and intestinal and systemic T cell differentiation was studied. Multiplex 454 pyrosequencing of fecal bacterial 16S rRNA was used to asses changes in composition of microbiota.

Results Oral treatment of arthritic IL-1Ra-/- mice with scGoslcFos significantly suppressed the progression of arthritis. Gene expression of T-bet and RORγt, the Th1 and Th17-related transcription factors, in lymph nodes draining the arthritic joints was significantly reduced in the group receiving the scGoslcFos diet. Furthermore, dual-energy X-ray absorptiometry scanning revealed that a prebiotic diet containing scGoslcFos significantly improved bone mineral density and tended to increase bone mineral content in arthritic IL-1Ra-/- mice.

High-throughput pyrosequencing revealed a that scGoslcFos has a profound effect on relative abundance of bacteria in different taxa. The most notable alterations concerned a significant increase in Lactobacilli and a strong decrease in the genus Turicibacter.

Interestingly, intestinal gene expression of the Treg-related transcription factor FoxP3 as well as anti-inflammatory cytokine IL-10 were increased with scGoslcFos. Accordingly, small intestine lamina propria lymphocytes of mice receiving the scGoslcFos diet produced significant higher levels of IL-10 upon ex vivo stimulation with PMA and ionomycin. Production of IL-4 and IFNγ also tended to be increased, while production of TNFα, IL-6 and IL-17 was not affected by the prebiotic diet.

Conclusions Our data suggest that scGoslcFos suppresses arthritis progression, potentially through induction of anti-inflammatory cytokines such as IL-10 and IL-4. Suppression of disease progression using dietary intervention with prebiotic scGoslcFos may be applicable as a therapeutic approach to suppress autoimmune arthritis.

Disclosure of Interest None declared

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