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AB1037 Multiplex Biomarker Analysis of Synovial Fluid and Serum in Rheumatoid Arthritis
  1. A. Novikov1,
  2. E. Aleksandrova1,
  3. D. Karateev2,
  4. E. Lutchihina2,
  5. T. Popkova2,
  6. M. Cherkasova1,
  7. E. Nasonov2
  1. 1Laboratory of Immunology and Molecular Biology of Rheumatic Diseases
  2. 2V.A. Nasonova Research Institute of Rheumatology, Moscow, Russian Federation

Abstract

Background Analysis of synovial fluid (SF) in rheumatoid arthritis (RA) provides an opportunity to discover the complex of biomarkers involved in the inflammatory process.

Objectives To characterize synovial fluid biomarker profile in RA.

Methods We studied 53 pts with RA (>6 months disease duration, median and interquartile range (25th–75th percentile) of age 51 (43-61) years, 72% female). Cytokine analyses were performed with multiplex technology (Human Grp I Cytokine 27-plex panel for BioPlex 200), anti-CCP2, anti-MCV was measured by ELISA, IgM RF and CRP - by immunonephelometry.

Results SF levels of inflammation marker (CRP), pro- (IL-1β,-6,-15), anti- inflammatory (IL-1ra,-10,-13) and Th17 cytokines (IL-17), angiogenic factor (VEGF), chemokines (IL-8, IP-10, MCP-1) were significantly higher than in serum. CRP, IgM RF, anti-CCP2, anti-MCV, IL-1β, -1ra, -2, -4, -5, -7, -9, -10, -12, -15, eotaxin, G-CSF, GM-CSF, IFN-γ, MCP-1 showed a positive correlation their SF and serum concentration.

Conclusions Multiplex biomarker analysis of SF is powerful instrument to investigation immunopathogenic mechanism of RA. Serum levels of autoantibodies, inflammation markers, cytokines, chemokines and growth factors is able to reflect such in the microenvironment within the inflamed joints.

Disclosure of Interest None declared

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