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A1.21 In-depth characterisation of CD24highCD38high transitional human B cells reveals different regulatory profiles
  1. S Hillion1,
  2. Q Simon1,
  3. D Cornec1,
  4. R Mageed2,
  5. JO Pers1
  1. 1INSERM ESPRI 29, Immunology and Immunotherapy, CHRU Morvan, Brest, France
  2. 2William Harvey Research Institute, Queen Mary University of London, EC1M 6BQ, UK

Abstract

CD24high CD38high transitional B cells represent a key stage in the developmental pathway of B cell peripheral tolerance and functional maturation. These B cells have been widely ascribed regulatory functions. However, the phenotypic and functional overlap between these cells and regulatory B cells remains controversial. In this study, we use multi-colour flow cytometry with bioinformatic analyses and functional studies to show that CD24high CD38high B cells can be differentiated into multiple subsets. The study also reveals for the first time that human transitional B cells encompass transitional type 1 (T1) and T2 B cells but also distinct anergic T3 B cells as well as IL-10-producing CD27+ transitional B cells. Interestingly, the latter two subsets differentially regulate CD4+ T cell proliferation and polarisation towards Th1 effector cells. Additional analyses show that the percentage of T3 B cells is reduced while the frequency of CD27+ transitional B cells is increased in patients with autoimmune diseases compared with matched healthy individuals. This study provides evidence for the existence of different transitional B cell subsets each displaying unique phenotypic and regulatory functional profiles.

Furthermore, the study indicates that altered distribution of transitional B cells subsets highlights different regulatory defects in autoimmune diseases

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