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A8.4 IL4–10 synerkine: a novel anti-inflammatory drug to inhibit immunopathology in inflammatory rheumatic diseases
  1. SAY Hartgring1,2,
  2. C Steen-Louws1,
  3. M De Smet2,
  4. CE Hack1,2,
  5. JAG van Roon1,2
  1. 1Department of Rheumatology and Clinical Immunology, UMCUtrecht, Utrecht, The Netherlands
  2. 2Laboratory of Translational Immunology, UMCUtrecht, Utrecht, The Netherlands

Abstract

Background A considerable percentage of patients shows a limited response to biologics targeting one specific inflammatory mediator, largely because of redundancy of these mediators. An attractive alternative is inhibition of multiple proinflammatory mediators and induction of immunoregulatory activity by regulatory cytokines such as IL4 and IL10. Many studies demonstrated the strong capacity of IL4 and IL10 as stand-alone drugs to inhibit inflammation and tissue-destructive responses in animal and human in vitro models. Clinical results of these cytokines, however, have been modest, possibly because of poor bioavailability that is mainly due to low molecular weight and rapid renal clearance.

Objectives To execute a feasibility study to develop IL4 and IL10 as one biologic (called IL4–10 synerkine), preserving distinct characteristics of each molecule plus improving bioavailablity by increasing the molecular size.

Materials and methods Biochemical properties of IL4–10 synerkine were determined by western blot, size exclusion chromatography, and ELISA. Functional properties were studied by measuring the capacity of IL4–10 synerkine to regulate production of pro-inflammatory cytokines and their inhibitors, as well as proinflammatory and regulatory T-cell activity, and expression of Fc receptors. In addition, blockade of IL4 and IL10 receptor was performed to confirm the specific activities of IL4 and IL10.

Results IL4–10 synerkine appeared as a glycosylated dimeric protein with a molecular size of ˜70 kDa, consisted of intact IL10 and IL4 subunits. In whole blood assays IL4–10 synerkine robustly and dose-dependently inhibited multiple pro-inflammatory cytokines, which was almost complete at 20 ng/ml (IL1β, TNFα, IL6 and IL8, all p < 0.001). This effect was dependent on interaction with IL10R and IL4R. Oppositely, the synerkine significantly induced production of IL1RA and preserved sTNFR levels. IL4–10 synerkine strongly inhibited Th1 and Th17 cytokine secretion (P < 0.01), while maintaining FoxP3 expression. Finally, while IL4 upregulated FcεR expression and IL10 upregulated expression of activating FcγRs on monocytes (all p < 0.001), both Fcε and FcγR expression were largely preserved at control levels in the presence of IL4–10 synerkine (all at least p < 0.01).

Conclusions IL4–10 synerkine is a novel anti-inflammatory drug that shifts multiple proinflammatory pathways towards immunoregulation. The increased molecular mass predicts better bioavailabilty in humans than the wild-type molecules, which potentially enhances its clinical efficacy. The strong and improved inhibitory activities of IL4–10 synerkine (as compared to IL4 and IL-10 monotherapy) that we observed in several animal models for inflammatory pain underscores the potential for treatment of inflammatory and possibly degenerative rheumatic diseases.

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