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A1.9 Among patients with venous thrombo-embolism, B cell subset disturbances characterise those with primary antiphospholipid syndrome
  1. L Simonin1,2,3,
  2. E Pasquier3,
  3. C Leroyer3,
  4. D Cornec1,4,
  5. B Bendaoud1,2,
  6. S Hillion1,2,
  7. F Couturaud3,
  8. Y Renaudineau1,2
  1. 1INSERM ESPRI, ERI29, EA2216, Brest University, and LabEx IGO, Brest, France
  2. 2Laboratory of Immunology and Immunotherapy, Brest University Medical School Hospital, Morvan, Brest, France
  3. 3Department of Internal Medicine and Lung Speciality, EA3878, IFR148, Brest University Medical School Hospital, Cavale Blanche, Brest, France
  4. 4Unit of Rheumatology, Brest University, Medical School Hospital, Cavale Blanche, Brest, France

Abstract

Background and objectives Lymphocytes subset distribution is altered in numerous autoimmune diseases, and our aim was to evaluate lymphocyte distribution in well-characterised primary antiphospholipid syndrome (pAPS) patients selected from a venous thromboembolism (VTE) cohort.

Materials and methods Cases of pAPS VTE patients (n = 11) and matched non-APS VTE patients were selected during their follow-up from the data base EDITH (study of determinants interactions of venous thrombosis), which is a monocentric case-control cohort (n = 5,539). In addition, matched autoimmune disease patients (Sjögren’s syndrome and systemic lupus erythematosus) and controls were also included. Using a 4 colours flow cytometer, peripheral blood B cell subsets (B1, transitional, naïve, and memory), T cell subsets (CD3, CD4, CD8) and NK-cells were explored.

Results In contrast to non-APS VTE patients and controls, pAPS VTE patients displayed total CD3 and CD8 naïve (CD45RA+CD27+) T cell reduction, and disturbance in B cell homeostasis with increased proportions of B1 cells, transitional B cells (CD24+ +CD38+ +) and naïve B cells, while memory B cells were reduced. The unswitch memory B cell percentage (IgD+CD27+), and even better the naïve (IgD+CD27-)/unswitched memory B cell ratio were both effective to distinguish pAPS VTE patients from non-APS VTE patients, and from patients with autoimmune diseases. Moreover, the absolute number of CD4 T cells was positively correlated with IgG anti-cardiolipin antibody levels.

Conclusions Disturbances of B cell homeostasis characterised, within VTE patients, those with pAPS during their follow-up and an elevated naïve/unswitched memory B cell ratio represent a useful biomarker to characterise them.

Keywords
  • venous thromboembolism
  • antiphospholipid syndrome
  • autoimmunity
  • NK-cells
  • B-cells.

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