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A cell-based bioassay for circulating bioactive IL-17: application to destruction in rheumatoid arthritis
  1. Ndiémé Ndongo-Thiam,
  2. Pierre Miossec
  1. Immunogenomics and Inflammation Research Unit and the Department of Clinical Immunology and Rheumatology, Hospices Civils de Lyon, EA 4130 University of Lyon 1, Hôpital Edouard Herriot, Lyon, France
  1. Correspondence to Professor P Miossec, Department of Clinical Immunology and Rheumatology, Hôpital Edouard Herriot, Lyon 69437, Cedex 03, France; miossec{at}univ-lyon1.fr

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Interleukin 17 (IL-17) plays an important role in many chronic inflammatory diseases such as psoriasis, psoriatic arthritis, ankylosing spondylitis and rheumatoid arthritis (RA).1 Measuring circulating IL-17 levels could be crucial to identifying patients who would benefit from IL-17-targeted therapies.2 Detection by ELISA has been difficult with high variability between studies. Furthermore, IL-17 function is critical to taking into consideration the contribution of activators (eg, tumour necrosis factor (TNF)-α acting in synergy with IL-17) and inhibitors (IL-25, autoantibodies).

In previous studies, human umbilical vein endothelial cells (HUVEC) were shown to be highly sensitive to IL-17 stimulation for the production of the chemokine IL-8.3 These cells were thus used in a functional bioassay to measure circulating bioactive IL-17. To determine the contribution of IL-17 in the IL-8-inducing activity, plasma samples at the dilution of 10% were first preincubated with or without an anti-IL-17 antibody at 10 µg/mL (R&D systems, Paris, France), before being added to HUVEC (see more details in figure 1 legend and in figure 1A, B). After 48 h of incubation, IL-8 production was measured in supernatants …

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