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Interleukin 17 (IL-17) plays an important role in many chronic inflammatory diseases such as psoriasis, psoriatic arthritis, ankylosing spondylitis and rheumatoid arthritis (RA).1 Measuring circulating IL-17 levels could be crucial to identifying patients who would benefit from IL-17-targeted therapies.2 Detection by ELISA has been difficult with high variability between studies. Furthermore, IL-17 function is critical to taking into consideration the contribution of activators (eg, tumour necrosis factor (TNF)-α acting in synergy with IL-17) and inhibitors (IL-25, autoantibodies).
In previous studies, human umbilical vein endothelial cells (HUVEC) were shown to be highly sensitive to IL-17 stimulation for the production of the chemokine IL-8.3 These cells were thus used in a functional bioassay to measure circulating bioactive IL-17. To determine the contribution of IL-17 in the IL-8-inducing activity, plasma samples at the dilution of 10% were first preincubated with or without an anti-IL-17 antibody at 10 µg/mL (R&D systems, Paris, France), before being added to HUVEC (see more details in figure 1 legend and in figure 1A, B). After 48 h of incubation, IL-8 production was measured in supernatants …
Footnotes
Contributors NN-T: experiments and writing; PM: concept and writing.
Funding NN-T is supported by the IHU prometteur OPERA. PM is a senior member of and supported by the Institut Universitaire de France.
Competing interests NN-T and PM hold a patent on the determination of bioactive IL-17.
Patient consent Obtained.
Ethics approval Ethics committee of the hospitals of Lyon.
Provenance and peer review Not commissioned; externally peer reviewed.