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AB0450 Myeloid Dendritic Cells and Plasmacytoid Dendritic Cells as Possible Target for the Immunotherapy in Patients with Rheumatoid Arthritis
  1. S.A. Falaleeva1,
  2. V.V. Kurilin1,
  3. N.S. Shkaruba2,
  4. O.A. Chumasova2,
  5. A.E. Sizikov2,
  6. S.V. Sennikov1
  1. 1Laboratory of Molecular Immunology
  2. 2Rheumatology department, Federal State Budgetary Institution “Research Institute of Clinical Immunology” under the Russian Academy of Medical Sciences Siberian Branch, Novosibirsk, Russian Federation

Abstract

Background Recently, dendritic cells (DC) are considered as an object to create a new therapeutic strategy in rheumatoid arthritis (RA), since they are involved in all stages of the inflammatory process. States of phenotypic and functional characteristics of DCs will depend on the possibility of their use as a therapeutic modulator and the targeted object to other biological treatment strategies [1].

Objectives To research the phenotypic and functional properties of myeloid (mDC) and (pDC) plasmacytoid DC in peripheral blood (PB) of patients with RA.

Methods Blood samples were obtained from 10 patients during the exacerbation of RA. Analysis of the DC target populations contents was performed using flow cytometer “BD FACS Aria”. Phenotypic characterization of immature mDC (Lin-HLA-DR+CD123-CD11c+) and pDC (Lin-HLA-DR+CD123+CD11c-) in the whole blood was estimated by the positive expression of CD80, CD83, CCR7, IL-10, IL-4, IL-12, IFN-α. To determine the ability of DC to mature stimulants R848 and lipopolysaccharide (LPS E.colli 0114: B4) were used.

Results The relative content of PB in the RA patients mDC (1%) and healthy donors (1.02%) did not differ, then the amount of pDC in RA patients (0.1%) was significantly lower than in healthy donors (0.6%). DC of RA patients have low levels of expression of CD80 CD83, both the mDC and pDC. In RA patients on pDC, an increase in the expression of CCR7 (62%) compared with normal (30.5%). In the PB of RA patients compared with healthy donors, shows the increased IL- 12, IFN-α and decreased IL- 4 and IL- 10 expression mDC and pDC [2].

Conclusions In PB of RA patients formation of unbalance as in cytokines as well in subsets of dendritic cells is observed. DC of patients with RA are characterized by less mature state wherein pDC have higher migration potential compared to healthy donors. Estimated data may lead to development of DC-targeting therapy in autoimmune diseases.

References

  1. Prevosto C., Goodall J.C., Hill Gaston J.S. Cytokine secretion by pathogen recognition receptor-stimulated dendritic cells in rheumatoid arthritis and ankylosing spondylitis. J.Rheumatol. 2012; 39(10): 1918-28.

  2. Van Krinks C.H., Matyszak M.K., Gaston J.S. Characterization of plasmacytoid dendritic cells in inflammatory arthritis synovial fluid. Rheumatology (Oxford). 2004; 43(4): 453-460.

Acknowledgements Work is supported by grants of the FTP “Scientific and Scientific-Pedagogical Personnel of the Innovative Russia in 2009-2013” (Agreement No. 8790)

Disclosure of Interest None declared

DOI 10.1136/annrheumdis-2014-eular.1136

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