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AB0176 Association between Polymorphisms in Beta-Adducin and Sodium/Calcium Exchanger 1 and SLE with and without Nephritis
  1. G.A. Ramirez1,2,
  2. E.P. Bozzolo2,
  3. N. Casamassima3,
  4. C. Lanzani3,
  5. P. Manunta1,3,
  6. V. Canti1,2,
  7. P. Rovere-Querini1,2,
  8. M.G. Sabbadini1,2,
  9. A.A. Manfredi1,2
  1. 1Università Vita-Salute S. Raffaele, Milan, Italy
  2. 2Department of Immunology
  3. 3Department of Nephrology, Ospedale San Raffaele, Milano, Italy

Abstract

Background Genes involved in cytoskeletal assembly and water/electrolyte balance have been previously linked to hypertension, but recent evidences suggest a role also in the development of inflammation and autoimmunity [1-2]. Systemic Lupus Erythematosus is a prototypic multi-organ autoimmune disease, characterized by polymorphic clinical features and a complex polygenic background. Renal disease in SLE occurs in 40-70% of lupus patients and is responsible of an high burden of morbidity and mortality. Despite substantial efforts, less is known about the role of specific genetic factors in conferring susceptibility to SLE and its complications, including lupus nephritis (LN) [3].

Objectives With these premises we sought if an association between known hypertension-related polymorphisms and development of SLE and LN existed.

Methods 106 patients (96 females, 10 males) diagnosed with SLE (n=51) or LN (n=55), 23 patients with Sjoegren's Syndrome and 62 healthy controls were enrolled. Patients and controls were genotyped for polymorphisms in α-, β- and γ-adducin (ADD1,2,3 respectively), angiotensin converting enzyme, transient receptor potential cation channel-subfamily C, solute carrier family 8 (sodium-calcium exchanger) member 1 (SLC8A1), solute carrier family 24 (sodium/potassium/calcium exchanger) member 3, PKD2 calcium channel and PRKG1 kinase genes.

Results Beta-adducin (ADD2) rs4984 CT heterozygosis was significantly more frequent in SLE patients than in LN patients (χ2 =6.154; p=0.013). No patient had the ADD2 rs4984 TT genotype in accordance with a low prevalence of the ADD2 C1797T homozygosity in the population. Seven patients with SLE (n=3) or LN (n=4), but none of the control groups carried the AA variant of sodium/calcium exchanger 1 (SLC8A1) rs11893826 SNP (χ2 =11.771; p=0.019).

Conclusions Genes involved in the control of electrolyte/water balance and cytoskeletal plasticity could affect the pathogenic background of SLE/LN. Larger studies are required to confirm these data and attempt correlations between genetic data and specific clinical features.

References

  1. Tintinger GR, et al., Drug Des Devel Ther, 2009

  2. Kleinewietfeld M, et al., Nature, 2013

  3. Ramos PS, et al., Semin Nephrol, 2010

Disclosure of Interest None declared

DOI 10.1136/annrheumdis-2014-eular.3378

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