Article Text

PDF
AB0155 Periostin Deficiency Aggravates Inflammation and Joint Destruction in an Animal Model of Rheumatoid Arthritis
  1. Y.S. Suh1,
  2. H.-O. Kim1,
  3. I.-I. Lee1,
  4. H.S. Lim1,
  5. Y.-H. Cheon1,
  6. W.-H. Yoo2,
  7. W.-S. Lee2,
  8. J.-M. Kim3,
  9. S.-H. Kim3
  1. 1Internal Medicine, Gyeongsang National University School of Medicine, Jinju
  2. 2Internal Medicine, Chonbuk National University Medical School, Jeonju
  3. 3Internal Medicine, Keimyung University, Daegu, Korea, Republic Of

Abstract

Background Periostin is a matricellular protein that expressed in bone, periodontal ligament, heart valve, tendon and skin. Previous study indicated that periostinis linked with several inflammatory diseases such as airway hypersensitivity, periodontal inflammation, and pulmonary fibrosis. However, there is no study to investigate the role of periostin in rheumatoid arthritis (RA).

Objectives This study was performed to assess the function of periostin in K/BxN serum transfer arthritic mice.

Methods Synovial tissues, serum, joint fluid, and fibroblast-like synoviocyte (FLS) were obtained from patients with RA and osteoarthritis (OA). The expression of periostin in synovial tissues was assessed by immunohistochemistry. The levels of periostin were checked by Western blot and enzyme-linked immunosorbent assay (ELISA). K/BxN serum transfer arthritis was induced in periostin knockout (KO) and age-matched wild type (WT) mice. Arthritis severity was assessed by clinical and histopathologic scoring. The levels of inflammatory cytokines in ankles and serum were measured by quantitative PCR and ELISA. The osteoclastogenesis was assessed by using bone marrow monocytes (BMM) from periostin KO or WT mice.

Results Periostin was highly expressed in the synovium of RA patient than OA. The periostin levels were increased in the presence of IL-1β and TNF-α in RA-FLS. The periostin KO mice showed more severe arthritis from day 4 to day 10. The peak clinical scores were: 5.0±0.6 in WT, 7.36±0.5 in periostin KO mice; p<0.01). The periostin KO mice showed aggravated histopathologic findings than WT, respectively: synovial inflammation (3.5±0.6 vs 2.2±0.4; p<0.05), bone erosion (3.8±0.6 vs 1.9±0.5; p<0.05) and cartilage damage (3.2±0.7 vs 1.8±0.5; p<0.05). The level of IL-1b was increased in the serum of periostin KO mice than WT (18 pg/ml ±7.0 vs 5 pg/ml ±1.2; p<0.01). BMMs from periostin KO mice had more TRAP-positive multinucleated cells than WT.

Conclusions This study suggests that regulation of periostin contributes to pathogenesis of RA and periostin has a potential protective role in RA.

Disclosure of Interest None declared

DOI 10.1136/annrheumdis-2014-eular.4201

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.