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AB0015 Association of BLK Polymorphism with Susceptibility to Rheumatoid Arthritis in an Algerian Population
  1. R. Djidjik1,
  2. I. Allam1,
  3. S. Louahchi2,
  4. N. Raaf2,
  5. N. Ouikhlef2,
  6. A. Boucharef2,
  7. A. Abdessemed3,
  8. N. Khaldoun3,
  9. N. Behaz2,
  10. K. Cherguelaine2,
  11. A. Tahiat2,
  12. A. Ladjouze Rezig3,
  13. M. Ghaffor2
  1. 1CHU Beni Messous Alger
  2. 2CHU Beni Messous
  3. 3EHS Ben Aknoun, Algiers, Algeria

Abstract

Background The B-cell lymphocyte kinase (BLK) is a src family protein tyrosine kinase expressed in B-lineage cells. BLK gene polymorphism was reported as a risk factor in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) susceptibility.

Objectives To investigate whether the association of BLK gene polymorphism with RA is present in an Algerian population.

Methods The BLK (rs13277113) single nucleotide polymorphism was directly genotyped in 223 RA patients and 200 healthy controls by real time -polymerase chain reaction method (TaqMan Assays). The relationships between anti-citrullinated peptide antibody (ACPA) positivity, rheumatoid factor (RF) positivity and BLK genotypes were analyzed statistically. Disease activity score (DAS28) and HAQ score were also assessed.

Results Significant associations between RA and controls were observed for AG heterozygote genotype (21% vs 33%, P=0.004, OR=0.53) and recessive model (AA vs. AG+GG, 94% vs 99%, P=0.002, OR=0.08). Allele frequencies did not differ between patients and controls and between antibodies positive (ACPA, RF) and negative RA (P>0.05). No significant difference in genotypes distribution was found after stratification according to parameters of disease activity (DAS 28 and HAQ, P>0.05).

Conclusions Our result indicates that polymorphism in BLK gene is associated with susceptibility to RA in Algerian population but not with the severity.

References

  1. Génin E, Coustet B, Allanore Y, et al. Epistatic interaction between BANK1 and BLK in rheumatoid arthritis: results from a large trans-ethnic meta-analysis. PLoS One. 2013 Apr 30;8(4)

  2. Viatte S, Plant D, Bowes J, et al. Genetic markers of rheumatoid arthritis susceptibility in anti-citrullinated peptide antibody negative patients. Ann Rheum Dis. 2012 Dec;71(12)

  3. Zhang H, Wang L, Huang Y, et al. Influence of BLK polymorphisms on the risk of rheumatoid arthritis. Mol Biol Rep. 2012 Nov;39(11).

Disclosure of Interest None declared

DOI 10.1136/annrheumdis-2014-eular.1943

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