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AB0811 Total Antioxidant Status and Oxidative Stress in Patients with Osteopenia and Osteoporosis
  1. K.S. Abdullah,
  2. D.A. Alhamdany
  1. Pharmacology - Rheumatology, Mosul Universtiy, Mosul, Iraq

Abstract

Background Osteoporosis (OP) is the most common metabolic bone disorders. OP results from an imbalance between bone formation and resorption in favor of the later1. Increased oxidative stress (OS) in bone enhances osteoblast and osteocyte apoptosis2.

Objectives To assess total antioxidant status and oxidative stress and in patients with osteopenia and osteoprosis.

Methods Patients: Sixty female patients with positive Dual-Energy X-Ray Absorptiometry (DEXA) results were enrolled in the study and were divided into 2 groups, osteopenia and osteoporosis. Control: Thirty apparently healthy females, matched for age with the patients group, with normal DEXA results were kept as control group.

Methods Dual-Energy X-Ray Absorptiometry (DEXA): Bone mineral density was obtained at lumbar vertebrae in all subjects using dual energy x-ray absorptiometry. The biochemical analysis: Total antioxidant status was measured using Randox TAS kit – UK). Serum MDA level was measured using thiobarbiturc acid (TBA) assay. Pilot study: A pilot study was designed to compare the TAS and oxidative stress by measurement of MDA between ostepenia and oseoporosis, using the ranges between the medians of each groups. The median of osteopenia is -1.75, therefore the ranges between -1.75 and -2.5 will be compared with the ranges of osteoporosis from -2.6 and -3.3 the median of osteoporosis score.

Results The TAS values of both patients groups (1.59±0.15 mmol/l and 1.05±0.37 mmol/l, respectively) were significantly lower than that of the control group (2.18±0.26mmol/l) (p<0.001). MDA values of both patients groups (osteopenia and osteporosis) (1.20±0.43 μmol/l and 2.40±0.66 μmol/l, respectively), were significantly higher than that of the control group (0.47±0.14 μmol/l) (p<0.001).

The result of pilot study, showed no difference between these two selected values groups for TAS and MDA, (p-value <0.1and 0.07) respectively.

Conclusions Patients with OP have lower levels of TAS and higher levels of MDA than the control group, and no difference in these parameters between osteopenia and osteoporosis in the pilot study.

References

  1. Raisz LG. Pathogenesis of osteoporosis: concepts, conflicts, and prospects. J Clin Invest 2005; 115(12):3318-25.

  2. Almeida M, Han L, Ambrogini E, Weinstein RS, Manolagas SC Glucocorticoids and tumor necrosis factor α increase oxidative stress and suppress Wnt protein signaling in osteoblasts. J Bone Miner Res 2011; 26(11):2682-94.

Disclosure of Interest None declared

DOI 10.1136/annrheumdis-2014-eular.2297

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