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AB0806 Polymorphisms Analysis of Bone Metabolism Genes in Relation to Bone Mineral Density in Patients with Chronic Periodontitis
  1. T. Lakhdar1,
  2. R. El jaoudi2,
  3. A. Ibrahimi3,
  4. O.K. Ennibi4,
  5. F. Znat1,
  6. F. Allali1,5,6,
  7. H. Rkain1,7,8,
  8. N. Hajjaj-Hassouni1,5,6
  1. 1Department of Rheumatology, El Ayachi University-Hospital, Rabat-Salé
  2. 2Laboratory of Pharmacology and Toxicology
  3. 3Médical Biotechnologie lab (MedBiotech), Faculty of Medicine and Pharmacy, University Mohammed Vth Souissi
  4. 4Department of Periodontology, Dental School, Mohammed V Souissi University
  5. 5Laboratory of Information and Research on Bone Diseases (LIRPOS-URAC 30)
  6. 6Laboratory of Biostatistical, Clinical Research and Epidemiology (LBRCE)
  7. 7Laboratory of Information and Research on Bone Diseases (LIRPOS-URAC 30)
  8. 8Laboratory of Physiology, Faculty of Medicine and Pharmacy, University Mohammed Vth Souissi, Rabat, Morocco

Abstract

Background Polymorphisms in Vitamin D receptor (FokI (VDR-F) and BsmI (VDR-B)), Estrogen receptor (Pvu II (ESR1P) and Xba I (ESR1X)), Collagen type1 (SpI (COL1A1) and Calcitonin Receptor (Alu I (CTR)) are thought to be candidate markers for osteoporosis.

Based on the fact that osteoporosis and periodontal diseases are bone destructive diseases, we investigated the relationship between polymorphisms in these candidate genes and bone mineral density (BMD) of lumbar spine and femoral neck in patients suffering from chronic periodontitis (CP).

Methods Lumbar spine and femoral neck BMD of individuals included in this study were diagnosed using the dual X-ray absorptiometry method. Blood was drawn and Genomic DNA was prepared and genotyped using MetaBone Clinical Arrays kit (Genomica, Spain). The genotyping and polymorphisms identification consisted on the simultaneous amplification of five regions of the genome containing the six SNPs of interest.

Clinical attachment level (CAL), gingival bleeding index (GI), and probing depth (PD) and Simplified Oral Hygiene Index (OHI-S) were determined in all participants by a single experimented examiner.

Results Table 1 (below) shows SNPs and variant alleles prevalence of genes associated with metabolic bone disorders in 18 chronic periodontitis patients:

Table 1

Statistical analysis showed that women with CP carrying the genotype VDRF-FOKI (Ff) have a lower lombar BMD (p=0.003) and higher number of teeth loss (p=0.003).

However, we were not able, in this preliminary study, to show any association between BMD of lumbar spine L2–L4 and Ward's BMD in CP patients and polymorphisms in different geotyped genes; Vitamin D receptor BsmI (VDR-B), Estrogen receptor PvuII (ESR1P), Collagen type1 SpI (COL1A1) and Calcitonin Receptor Alui (CTR). Furthermore, no association was found between these polymorphisms and severity parameters of chronic periodontitis.

Conclusions This preliminary study showed that Lower lombar BMD were observed in patients who carried VDRF-FOKI Ff genotype and lower prevalence of normal variants of VDRB-BSMI, CTR-ALUI and ESR1X-XBAI in the population of chronic periodontitis women. A larger case control study is ongoing in order to get bigger data to complete this report.

Disclosure of Interest None declared

DOI 10.1136/annrheumdis-2014-eular.5961

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