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A8.22 The role of proinflammatory and anti-inflammatory cytokines on CD3ζ-chain expression of human T- lymphocytes
  1. Nikolett Marton1,
  2. Barbara Molnár-Érsek1,
  3. Edit Buzás1,
  4. György Nagy1,2
  1. 1Department of Genetics, Cell, and Immunobiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary
  2. 2Department of Rheumatology, Faculty of Medicine, Semmelweis University, Budapest, Hungary

Abstract

Background ζ-chain has an important role in T-lymphocyte activation, and decreased ζ-chain expression is common at the sites of chronic inflammation. The precise mechanism of the ζ-chain downregulation is not known. Our earlier data suggested that CD3 ζ-chain expression of T-lymphocytes isolated from patients with rheumatoid arthritis, was lower than that of the healthy donors (p <0.05), and tumor necrosis factor (TNF) modulated T cell activation during inflammation by regulating the amount of CD3ζ-chain expression via a Src-like adaptor protein (SLAP) dependent mechanism. Our objective was to study the effect of proinflammatory and anti-inflammatory cytokines on the ζ-chain expression.

Materials and Methods PBMCs were separated from whole-blood samples by density gradient centrifugation. Jurkat cells and PBMCs were cultured in RPMI supplemented with 10% fetal bovine serum. PBMCs were activated with anti-CD3 and anti-CD28 antibodies. Cells were treated with 20 and 80 ng/ml TNF, IL- 1, IL-2, IL-4, IL-6, IL-8, IL-10; IL-13, IL-17 and IL-35 for 24 hours or left untreated. ζ-chain expression was measured by Western blot and by flow cytometry.

Results IL-6, IL-8, IL-10, IL-13 and IL-17 downregulated CD3ζ-chain expression (p<0.05), similarly to TNF.

Conclusion Our present data indicate that both proinflammatory and anti-inflammatory cytokines may have a role on T-cell activation through regulating CD3ζ-chain expression.

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