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A3.20 The calcium sensor stromal interaction molecule 1 (STIM1) controls regulatory B cell functions and its activity is impaired in Systemic Lupus Erythematosus patients
  1. T Fali1,
  2. Y Renaudineau1,
  3. M Burgos2,
  4. E Bariller1,
  5. S Jousse1,
  6. A Saraux1,
  7. C Hanrotel1,
  8. D Cornec1,
  9. C Jamin1,
  10. O Mignen2,
  11. J O Pers1
  1. 1EA2216 Immunology, Pathology and Immunotherapy, Brest University Medical School, Brest, France
  2. 2U1078 Canalopathy and Calcium Channels, Brest University Medical School, Brest, France

Abstract

Background and Objectives The immunosuppressive function of regulatory B cells (Breg) is defective in B cells from systemic lupus erythematosus (SLE) patients. Since the Ca2+ pathway is also altered in SLE B cells, the aim of the study was to explore the contribution of the Ca2+ channel Orai1 and its regulator, the stromal interaction molecule 1 (STIM1), on regulatory B cell functions.

Materials and Methods Thirty SLE patients and healthy controls (HC) were included in the study. Orai1 and STIM1 expressions were explored in CD40/CpG activated B cells, and in the stimulated (anti-CD3, anti-CD28 plus CpG-ODN 2006) T- and B- cell autologous co-culture Breg model. Transfection were used using either siRNAs to down-modulate STIM1 in SLE B cells, or either the cDNA STIM1 vector in HC B cells.

Results After 3 days, in CD40/CpG activated HC B cells, and in HC B cells stimulated during co-cultures, acquisition of the Breg phenotype (IgD/CD38/CD24/CD5high) was associated with an over-expression of the Ca2+ regulator STIM1 (x10.8 and x7.1 fold increase respectively). At day 4 and even more at day 5, STIM1 expression declined in co-cultures and this down-regulation was accompanied with IL-10 and TGF-beta up-regulation in B cells, FoxP3+ regulatory T cell expansion, and a reduction of T cell proliferation. Expression of the Ca2+ channel Orai1 was stable. In SLE B cells, STIM1 expression was overexpressed at basal level when compared to HC B cells (x4.3 fold) and remains elevated in B cells during all the time of the autologous co-culture (x8.8 fold). Then we hypothesised that maintaining high levels of STIM1 was critic in the regulatory capacity of SLE B cells. Accordingly, we demonstrated that STIM1 downregulation in SLE B cells, using a specific siRNA, was effective to restore IL-10, but not TGF-beta, expression, FoxP3+ regulatory SLE T cell expansion, and SLE T cell inhibition. In HC B cells, forcing STIM1 expression, with a STIM1 vector, was effective to reproduce the abnormalities observed in SLE B cells. No association was observed between STIM1 expression at basal level and organ involvement, disease activity (SLEDAI), or serological parameters thus suggesting that STIM1 over-expression and Ca2+ dysregulations are primary events in SLE.

Conclusions Altogether, this study reveals that acquisition of the Breg phenotype and Breg functions are tightly regulated by STIM1. Furthermore, this observation could provide innovative B-cell based treatment to convert B cells into immunosuppressive cells with applications in human autoimmunity and in SLE.

Key words lupus, regulatory B cells, calcium, STIM1, IL-10

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