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A1.82 Limited role of microrna 155 innate immunity dependent arthritis
  1. Eliana Goncalves-Alves1,
  2. Victoria Saferding1,
  3. Antonia Puchner1,
  4. Josef Smolen1,
  5. Kurt Redlich1,
  6. Stephan Blüml1
  1. 1Medical University Vienna, Dpt. of Rheumatology

Abstract

Background MicroRNA 155 (miR155) has been demonstrated to be essential for the development of collagen induced arthritis by controlling the generation of autoreactive T and B cells. However, the contribution of miR155 in innate immune cells is not known.

Materials and Methods We analysed activation and cytokine production of macrophages and dendritic cells (DCs) in vitro and in vivo. We analysed T-cells stimulatory capacity of DCs. We crossed miR155 deficient mice into hTNFtg mice and analysed arthritis development clinically as well as histologically.

Results MiR155 deficiency did not alter the differentiation of BMDC from bone marrow cells or the expression of costimulatory molecules or MHCII expression after stimulation of macrophages and DCs in vitro and in vivo. Proinflammatory cytokine production (IL-12p40, IL-6) after LPS challenge in vivo was not different in serum of wt mice and miR155-/-mice. We also Facs-sorted DCs after stimulation with LPS in vivo and determined the production of proinflammatory cytokines such as IL-23, IL-6 as well as TNF. We did not detect differences between wt and miR155-/- mice. In addition, the T cell stimulatory capacity of wt and miR155-/-was identical in vitro and in vivo. When we analysed miR155-/-mice compared to wt mice in the hTNFtg model of inflammatory arthritis, which is independent of the adaptive immune system, we did not detect differences in the clinical signs and symptoms of arthritis. Histologically, we even found slightly increased synovial inflammation in hTNFtg/ miR155-/-mice compared to wt mice.

Conclusion In contrast to the pivotal role of miR155 in autoimmunity requiring the adaptive immune system, the role of miR155 in innate immunity seems to be limited. This is emphasised by the fact that miR155 hardly influences the course of TNF-driven arthritis, which is mainly dependent on components of the innate immune system.

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