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1.64 Interleukin-33 suppresses experimental arthritis through promoting Foxp3+ regulatory T-cells and type-2 immune responses in mice
  1. Jérôme Biton1,
  2. Allan Thiolat1,
  3. Sara Khaleghparast Athari1,
  4. Delphine Lemeiter1,
  5. Roxane Hervé1,
  6. Stéphane Rogas3,4,
  7. Anais Levascot5,
  8. Patrice Decker1,
  9. Jean-Philippe Girard3,4,
  10. André Herbelin5,
  11. Marie-Christophe Boissier1,2,
  12. Natacha Bessis1
  1. 1INSERM UMR 1125 Sorbonne Paris Cité, Université Paris 13, 74 rue Marcel Cachin, 93000, Bobigny, France
  2. 2Assistance Publique-Hôpitaux de Paris, Hôpital Avicenne, Service de Rhumatologie, 125 rue de Stalingrad, 93000, Bobigny, France
  3. 3Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, F-31077 Toulouse, France
  4. 4Université de Toulouse, Université Paul Sabatier, Institut de Pharmacologie et de Biologie Structurale, F-31077 Toulouse, France
  5. 5INSERM U1082, Pôle Biologie Santé, 1, rue Georges Bonnet BP 633, 86022 Poitiers

Abstract

Background and Objectives Interleukin (IL)-33 is a new member of the IL-1 family that exerts pleiotropic activities in innate and adaptive immunity. With its receptor ST2, they have newly emerged as key molecules strongly involved in several inflammatory and autoimmune disorders. Recent evidence suggests that the IL-33/ST2 axis is strongly involved in the pathophysiology of rheumatoid arthritis (RA). However in RA models, the role of IL-33 and its receptor is still controversial. We aimed at deciphering IL-33 mode of action after administration in an experimental model of RA, namely collagen-induced arthritis (CIA).

Material and Methods CIA was induced by immunisation of C57Bl/6 mice with type 2 collagen. IL‑33 was ip administrated in CIA mice and cells were analysed by flow cytometry on day 28 after CIA induction.

Results We show a previously unshown dramatic inhibition of mouse collagen-induced arthritis (CIA) development after repeated administration of IL-33. This therapeutic effect was related to an enhanced type-2 immune response, including the expansion of eosinophils, Th2 cells, innate type 2 lymphoid cells (ILC2, defined as CD25+ c-Kit+ Lin- Sca-1+ST2L+) and an increase in Th2 cytokines levels in the serum of treated mice. Moreover, our work brings out the interplay between Treg and IL-33. Since IL-33 acts directly on Treg via ST2L, we showed that IL-33 treatment of CIA majors Treg frequency and increases the suppressive capacities of those cells. IL-33 also induces the emergence of a CD39+/high Treg population in a ST2L dependant manner.

Conclusions In the light of our present study, IL-33 can exert powerful anti-inflammatory properties in CIA, integrating the establishment of a type-2 immune response, the expansion and the activation of Treg. Our study reveals an undescribed mechanism by which IL-33 inhibits arthritis development, thus updating and strengthening the crucial role of IL-33 in RA.

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