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AB0687 Th-17 cytokine profile in children with juvenile idiopathic arthritis.
  1. J. Postępski1,
  2. K. Pogoda2,
  3. M. Pyszniak2,
  4. E. Olesińska1,
  5. B. Wilczyńska2,
  6. J. Tabarkiewicz2
  1. 1Department of Pediatric Pulmonology and Rheumatology
  2. 2Department of Clinical Immunology, Medical University, Lublin, Poland

Abstract

Background Th17 cells have recently been identified as important in pathogenesis of chronic arthritis including juvenile idiopathic arthritis (JIA). Th17 are highly proinflammatory subset of T cells, characterised by production of interleukin 17 (IL-17/IL-17A). Among members of the IL-17 family designated IL-17A–F, IL-17F is most closely related to IL-17A. IL-17A enhances inflammatory reactions, promotes expansion and recruitment of innate immune cells such as neutrophils. IL-17F induces non-immune cells such as epithelial cells or stromal cells to act proinflammatory. Th17 cells also secrete interleukin-21 (IL-21) and 22 (IL-22).

Objectives The aim of our study was to investigate the profile of Th17 cells producing IL-17A, IL-17F, IL-21, IL-22 in children with juvenile idiopathic arthritis (JIA).

Methods We examined peripheral blood (PB) of 22 patients with JIA (12- female, 9-male) mean age 11±0,7y. Patients were diagnosed according to the ILAR criteria. The type of onset was: oligoarticular – 8, polyarticular – 8, enthesitis related – 6. 8 children submitted to orthopedic surgery acted as a control. The proportion of Th17 cells producing IL-17A, IL-17F, IL-21, IL-22 was performed with multiparameter flow cytometry at two time points, before treatment and in remission.

Results In remission a significant decrease of Th17 compared to the acute phase was found. Analysis IL-17 isoforms of Th17 showed a significant decrease in lymphocytes producing IL-17F (IL-17A-/IL-17F +). There was also a decrease in lymphocytes IL-17A+/IL-17F+, but this result did not reach the level of significance (p = 0.08).

The proportion of cells producing only IL-17A remained unchanged. It is interesting that we found a significant decrease in the proportion of lymphocytes IL-17A+/IL-17F-/IL-21-/IL-22 +.

Conclusions The study shows that in JIA in remission the proportion of Th17 producing IL-17F significantly decreases. The proportion of cells producing only IL-17A remained unchanged, but with the exception of cells producing IL-22 which deceased in remission. In summary, our results would support the hypothesis that activity of Th17 pathway could correlate with proinflammatory activity of non-immune cells.

  1. Nistala K et al. Arthritis & Rheumatism 2008;58:875-887

  2. Omoyinmi E, et al. Rheumatology (Oxford). 2012;51(10):1881-1886

  3. Nistala K, et al. Proc Natl Acad Sci U S A. 2010;107:14751–14756

Disclosure of Interest None Declared

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