Background Several antibodies have proven useful in autoimmune diseases, as markers for diagnosis, prognosis or clinical manifestations.
Objectives To evaluate the clinical utility and the various associations of antibodies SSA/Ro52, SSA/Ro60 and SSB/La in LES at a referral hospital in Spain.
Methods We retrospectively analise the patterns of all the consecutive samples submitted to the Immunology Unit for antinuclear antibody screening between 2002 and 2012. ANA positive sera for indirect immunofluorescence test (IFI) were analised with a commercial immunoassay (Innolia-ANA update). 323 patients had more than one positive sample for some of the autoantibodies Ro52, Ro60 or La and the pattern of sera for each patient were virtually identical. 113 patients with diagnosis of LES were included in the study. Dependent variables: Each of the possible combinations of results of Ro52, Ro60 and La, ie: 52+/60+/La+; 52+/60+/La-; 52+/60-/La+; 52+/60-/La-; 52-/60+/La+; 52-/60+/La-; 52-/60-/La+. Independent variables: age, sex, diagnostic variables, pattern by IIF, result of the immunoassay, clinical features and laboratory variables.
Results Of the 113 patients, 93,8% were women and 6,3% males, aged between 16 and 71 years (mean: 37,7; dev. Typical 13.9) in the moment of the diagnosis. The most frequent pattern were fine speckle, 68 cases (61,3%) and homogeneous 41 (36,6%)
The relation of clinical symptoms and the positivity of Ro52, Ro60 and La in their various combinations (Table 1) showed the following statistical association: xerostomia and rheumatoid factor + with Ro52+/Ro60-/La+; arthritis with Ro52+/Ro60+/La-; xerostomia, xerophthalmia, hypergammaglobulinemia and rheumatoid factor with isolated Ro52+; pericardial effusion, subacute cutaneous lesions and hypocomplementemia with Ro52-/Ro60+/La-. In relation to IFI, fine speckeled pattern was positively associated with Ro52+/Ro60+/La+ combination (p 0,003), and La+ isolated (p 0,03).
Conclusions Our study found significant relationship between clinical and laboratory manifestations and different patterns of antibodies to Ro52, Ro60 and SSB. The combination of antibodies might be clinically useful because these associations have prognostic and therapeutic implications. Would be worthwhile to explore its usefulness in other ethnic groups.
Disclosure of Interest None Declared
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