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AB0016 Association between the hla-b51 antigen and profiles of peripheral t cell subsets in tunisian patients with behcet’s disease
  1. K. Sakly1,
  2. C. Lambert2,
  3. M. Maatouk1,
  4. S. Hammami3,
  5. N. Sakly1,4,
  6. O. Harzallah3,
  7. S. Mahjoub3,
  8. I. Ghedira1,
  9. S. Feki1
  1. 1Research unit 03/UR/07 “autoimmunity and allergy”, Faculty of pharmacy, Monastir, Tunisia
  2. 2Laboratory of Immunology, University Hospital Saint Etienne, Saint Etienne, France
  3. 3Internal medicine, F.B. University Hospital
  4. 4Unity of Immunology of EPS Fattouma Bourguiba, Monastir, Tunisia

Abstract

Background Behçet’s disease (BD) is a multisystemic inflammatory disorder characterized by spontaneous remissions and relapses [1]. An association between Behçet’s disease and HLA-B51 has been regarded as the strongest evidence for involvement of genetic factors in its pathogenesis. The exact mechanisms illustrating the involvement of HLA-B51 in BD development are still unknown [2].

Objectives The aim of this study was to assess possible associations between the expression of HLA-B51 antigen and particular profiles of peripheral T cell subsets in patients with BD.

Methods 30 patients with BD, 19 males and 11 females (mean age: 39.65±10.7 years) were enrolled in this study. Ten of them had active disease. The following monoclonal antibody combination was used to characterize the phenotypes of T cells: anti-CD4-Chrome orange/anti-CD3-PacificBlue/anti-CD62L-PE/anti-CD127-APC-Cy7/anti-CD25-PerCP-Cy5/anti-CD45RA-PCy7 and anti-CD8-APC. Analysis was performed on a FACSDiva™ flow cytometer using FACSDiva™ software. The HLA-B51 typing was performed using the complement dependent microlymphocytotoxicity technique.

Results Our study showed that 38.46 % of patients with BD were HLA-B51 positive (positive group). This group, as compared with HLA-B51 negative patients (negative group), tend to have fewer CD45RA+CD62L+CD4+ naïve T cells (6.77 ± 4.7% versus 8.18 ± 6.5%, p=0.9 ) and higher CD45RA-CD62L+CD4+ central memory T cells (22.7 ± 13.6% versus 13.18 ± 12.2%, p=0.2). The HLA-B51 positive group showed also lesser terminally differentiated CD45RA+CD62L–CD4+ effector memory T cells than the negative group (8.18±3.1% versus 16.86 ± 5%; P = 0.018). However, regarding CD45RA–CD62L–CD4+ effector memory T cells and CD4+CD25brightCD127- T reg cells, no differences were found between the two groups. Moreover, the HLA-B51 positive group seems to be characterized by decreased percentage of CD45RA+CD62L+CD8+ naïve T cells compared to negative group (8.11±3.7% versus 14.02±6.7%; P=0.08). Finally, no differences were found between the two groups regarding CD45RA-CD62L+CD8+ central memory T cells, CD45RA–CD62L–CD8+ effector memory T cells and terminally differentiated CD45RA+CD62L– CD8+ effector memory T cells.

Conclusions Our study showed that HLA-B51 positive patients with BD are characterized by particular profiles of T cells. The understanding of these findings could contribute in elucidating the role of HLA-B51 antigen expression in the pathogenesis of BD.

References

  • Kaneko, F., et al., Behcet’s disease (Adamantiades-Behcet’s disease). Clin Dev Immunol, 2011. 2011: p. 681956.

Disclosure of Interest None Declared

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