Background The destruction of bone and cartilage tissue is one of the main manifestations of RA, which is related to the production of proinflammatory cytokines (TNF-α, IL-1 and IL-6) and making of cells synovium proteolytic enzymes (matrix metalloproteinases –MMP). The presence of anti-citrullinated proteins antibodies is also a risk factor of heavy destructive defeat of joints. Anti-MCV bound to the osteoclast surface, lead to a induction of osteoclastogenesis and bone resorption in vitro.
Objectives To evaluate the intercommunication between the level of anti-MCV and bone destruction in patients (pts) with RA.
Methods The study included 105 RA patients (85 women, mean age 53,0; 42,0-60,0, mean disease duration 60,0; 28,0-108,0 months, mean DAS 28 5,9; 5,2-6,7).
All pts received disease-modifying antirheumatic drugs (DMARD) (75,4% - methotrexate, 16,7% - leflunomide, and 7,9% - other DMARDs) and glucocorticoids (61,4%). Radiographs of the hands and feet was estimated by using van der Heijde-modified Sharp scores. Erythrocyte sedimentation rate – ESR (mm/hr) was determined by Westergren method; serum concentrations of anti-MCV (U/ml) and - MMP 3 (ng/ml) were tested using ELISA. The levels of IL-1β, IL-6 and TNF-α (pg/ml) were assayed by multiplex technology xMAP.
Results Mean [Me (interquartile range)] total modified Sharp score (mTSS) were 89 (58-116), erosion score - 9 (3-27), the level of anti-MCV- 470,2 (106,9-1000), IL-1β- 4,6 (2,1-10,2), IL-6 -125,6 (60,1 -370,7), TNF-α- 87,2 (39,4-391,2).
Depending on the level of anti-MCV all pts were divided into two groups. Among the high positive RA pts there were a higher value of mTSS and often detected elevated levels of MMP-3 (56%) compared to negative/low positive pts (31%, p=0,038), table 1. The level of proinflammatory cytokines was also higher in the first group (table 1). Significant differences between the groups according to the age, disease duration, activity, DMARDs therapy was not found.
Conclusions High positive level anti-MCV is associated with more pronounced destructive changes in the joints due to higher levels of MMP-3 and proinflammatory cytokines among this group of patients with RA.
Disclosure of Interest None Declared
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