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A2.13 Phenotypic and Molecular Profile of Innate Lymphoid Cells in Chronic Synovial Inflammation
  1. Hulda Sigridur Hreggvidsdottir1,2,
  2. Jenny Mjösberg2,
  3. Jochem Bernink2,
  4. Dominique Baeten1,
  5. Hergen Spits2
  1. 1Department of Clinical Immunology and Rheumatology
  2. 2Tytgat Institute for Liver and Intestinal Research, Academic Medical Center/University of Amsterdam, Amsterdam, The Netherlands

Abstract

Background and Objective Innate lymphoid cells (ILCs) represent a novel family of effector and regulatory cells in innate immunity and tissue remodelling. The family comprises several phenotypically and functionally distinct subsets that produce various cytokines such as IL-22, IL-17, IFNγ, TNF, IL-13 and IL-5, of which many have been shown to be important in arthritis pathogenesis.

The IL-17 and IL-22 producing ILCs are of major interest as they are implicated in chronic gut inflammation. Based on the broad clinical overlap between inflammatory bowel disease and spondyloarthritis (SpA) and the clinical importance of IL-17 in SpA we hypothesise that IL-17 and IL-22 producing ILCs contribute to inflammation and remodelling in SpA synovitis. As these cells have never been described in the joint our first aim was to characterise ILC in chronic inflammatory arthritis.

Material and Methods ILCs (lineage negative, CD45+CD127+) were analysed and sorted by flow cytometry from synovial tissue and fluid from rheumatoid arthritis (RA) and SpA patients as well as in blood from SpA patients and healthy donors. mRNA expression of sorted and expanded cells was analysed by qPCR.

Results ILCs were identified in blood as well as in synovial tissue and fluid from both RA and SpA patients. The frequency of ILCs was higher in the inflamed joint (0.5–3.3% of the lymphocyte population) than in the peripheral blood compartment (0.1%). In blood, there was no marked difference in the frequency of the different ILC subset between healthy controls (n = 10) and SpA patients (n = 5). In the inflamed joint, the ILC3 (CRTH2-NKp44+ckit+) and ILC1 (CRTH2-NKp44-ckit–) populations, previously shown to express IL-22 and IFNγ respectively in other tissues, were present in all samples whereas the Th2 cytokine expressing ILC2s (CRTH2+) were found in very low frequencies. Frequencies of ILC subpopulations varied considerably between patients and no differences could be detected between RA and SpA patients. qPCR analysis of expanded cells revealed that ILC1 expressed TBX21 whereas ILC3 expressed RORC. Accordingly, stimulated ILC3 expressed transcripts for both IL-23R and IL-22 but not IL-17.

Conclusions ILC1 and ILC3 are present in the chronically inflamed joint and express the key transcription factors associated with specific cytokine profiles. These data indicate that ILC could contribute to local cytokine-driven immune alterations in SpA and RA.

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