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A9.14 Regulatory T Cell Abnormalities in Patients with SLE Suggest an IL-2-Based Immunotherapy
  1. C von Spee1,2,
  2. N Wassermann2,
  3. JY Humrich1,2,*,
  4. G Riemekasten1,2,*
  1. 1German Rheumatism Research Centre, Berlin, Germany
  2. 2Charité University Hospital, Rheumatology and Clinical Immunology, Berlin, Germany.

Abstract

Background Phenotypic and quantitative abnormalities of regulatory T cells have been described in association with systemic lupus erythematosus (SLE). Further, an impaired production of IL-2, the essential cytokine for Treg homeostasis, has been described for T cells from SLE patients. Here, we aim to substantiate the link between IL-2 deficiency and Treg abnormalities in SLE and to provide the basis for an IL-2 based immunotherapy.

Methods Phenotype, frequency and homeostatic status of Foxp3+CD127lo Treg and conventional Foxp3- T cell (Tcon) subsets were analysed by multi-colour flow-cytometry of PBMCs from SLE patients and healthy donors ex vivo and after in vitro low-dose IL-2 treatment. Disease activity was determined according to the SLE activity index (SLEDAI). Two-tailed Mann-Whitney U test or 2-way ANOVA test were used for statistical analysis between patient or treatment groups, Spearman’s rank coefficient was used to calculate correlations with disease activity.

Results The frequency of CD25+ cells among Treg was significantly reduced in SLE patients compared to HC. Analysis of Ki67 expression revealed that proliferation was significantly increased in Tcon from SLE patients, resulting in a reduced Treg to Tcon proliferation ratio in SLE patients. The proliferation ratio correlated positively with the frequency of CD25+ Treg and inversely with disease activity. Treatment of SLE PBMCs with low-dose IL-2 in vitro resulted in increased frequencies of CD25+ cells among Treg and increased CD25 expression levels on Treg. Treg, but not Tcon proliferation was significantly increased under low-dose IL-2 treatment compared to untreated controls.

Discussion Similar to what has been observed in lupus-prone mice and IL-2-/- mice, Treg from SLE patients show the classical hallmarks of IL-2 deficiency with loss of CD25 expression and impaired homeostasis. Our in vitro results show that these Treg defects can be restored by low-dose IL-2 treatment, suggesting IL-2 as a novel therapeutic target for SLE.

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