Increased susceptibility of Trpv4-deficient mice to obesity and obesity-induced osteoarthritis with very high-fat diet
- 1Department of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina, USA
- 2Department of Biomedical Engineering, University of North Carolina–Chapel Hill, Chapel Hill, North Carolina, USA
- 3Program in Free Radical Biology and Aging, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA
- 4Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA
- Correspondence to Dr Farshid Guilak, Department of Orthopaedic Surgery, Duke University Medical Center, 375 MSRB, Box 3093, Durham, NC 27710, USA;
- Accepted 27 October 2012
- Published Online First 23 November 2012
Objective To test the hypotheses that: (1) the transient receptor potential vanilloid 4 (TRPV4) ion channel is protective in the obesity model of osteoarthritis (OA), resulting in more severe obesity-induced OA in Trpv4 knockout (Trpv4−/−) mice; and (2) loss of TRPV4 alters mesodermal stem cell differentiation.
Methods Male Trpv4−/− and wild-type (Trpv4+/+) mice were fed a control or high-fat diet (10% kcal and 60% kcal from fat, respectively) for 22 weeks, at which time spontaneous cage activity and severity of knee OA were evaluated. In addition, the adipogenic, osteogenic and chondrogenic potential of bone marrow-derived (MSC) and adipose-derived (ASC) stem cells from Trpv4−/− and Trpv4+/+ mice were compared.
Results A high-fat diet significantly increased knee OA scores and reduced spontaneous cage activity in Trpv4−/− mice, while also increasing weight gain and adiposity. MSCs from Trpv4−/− mice had decreased adipogenic and osteogenic differentiation potential versus Trpv4+/+ MSCs. ASCs from Trpv4−/− mice had increased adipogenic and osteogenic and reduced chondrogenic differentiation potential versus Trpv4+/+ ASCs.
Conclusions Pan-Trpv4−/− mice develop more severe OA with high-fat feeding, potentially due to more severe diet-induced obesity. The altered differentiation potential of Trpv4−/− progenitor cells may reflect the importance of this ion channel in the maintenance and turnover of mesodermally-derived tissues.
Obesity is one of the most significant and modifiable risk factors for osteoarthritis (OA).1 However, local biomechanical factors associated with changes in the onset and progression of knee OA in the obese population2 ,3 cannot explain the relationship between obesity and OA in non-load bearing joints.4 Obesity and related metabolic syndromes are associated with chronic low-grade inflammation and systemic tissue damage.5 Recent studies suggest that these systemic metabolic factors participate in the development of OA in both weight bearing and non-weight bearing joints;6 however, the mechanisms by which these systemic factors alter the course of OA remain unclear.7
The transient receptor potential vanilloid 4 (TRPV4) ion channel is a Ca2+-preferred cation channel, originally characterised as a transducer of osmotic stress.8 ,9 TRPV4-mediated Ca2+ signalling in response to osmotic fluctuations in the cartilage is one potential mechanism by which chondrocytes sense and respond to joint loading.10 Recent findings indicate that TRPV4 signalling plays a crucial role in skeletal development,11 ,12 while genetically-encoded deletion of TRPV4 in mice leads to accelerated joint degeneration with aging.13 More recent findings also suggest that chondrocyte TRPV4 could be a multi-modally modulated channel, interacting with pro-inflammatory mediators and cytokines to mediate catabolic signalling and nociception.14 ,15
We hypothesised that the absence of TRPV4-mediated signalling in the presence of the catabolic, biomechanical and inflammatory factors of obesity would accelerate OA progression in the high-fat diet model of OA. To examine the link between the observed phenotype of Trpv4−/− mice and function of TRPV4 at the cellular level, we measured the effects of TRPV4 deficiency on the intrinsic capabilities of bone marrow-derived stem cells (MSCs) and adipose-derived stem cells (ASCs), isolated from Trpv4−/− and Trpv4+/+ mice, to differentiate towards the adipogenic, osteogenic, and chondrogenic lineages.
Materials and methods
Detailed methods are available as online supplementary material.
At 10 weeks of age, male pan-Trpv4 knockout (Trpv4−/−) and wild-type (Trpv4+/+) mice were placed on either a high-fat (60% kcal) or control diet (10% kcal) for 22 weeks, at which time spontaneous locomotor activity was measured.
Immediately following sacrifice, total body fat of each mouse was measured using dual energy x-ray absorptiometry (DXA).6
Histological evaluation of OA
Sections of hind limb joints were stained with haematoxylin, Safranin-O and Fast Green and scored for degenerative changes using a modified Mankin system by three blinded graders.16 Sections of subcutaneous fat tissue from 10-week-old Trpv4−/− and Trpv4+/+ mice were also taken and stained with H&E.
Stem cell isolation, purification and expansion
MSCs and subcutaneous ASCs were isolated from the femurs and tibias (MSCs), and the inguinal fat pad (ASCs) of Trpv4−/− and Trpv4+/+ mice (8–10 weeks old), using a recently described method involving fluorescence-activated cell sorting (FACS) to obtain cells with specific cell markers,17 ,18 and expanded to P3.
Passage 3 MSCs and ASCs were induced towards the adipogenic, osteogenic or chondrogenic lineages and assayed for differentiation capacity. Adipogenesis was quantified by Oil-Red-O release, while osteogenesis was quantified by Alizarin stain release. Chondrogenic differentiation was assessed by Alcian blue staining and glycosaminoglycan (GAG) content.
Normality was tested, and data were log-transformed before analysis if necessary. Statistical analysis was performed using multiple-factor analysis of variance for comparison of multiple groups, with Fisher Least Significant Difference (LSD) post-hoc analysis using α=0.05. Significant differences were reported at the 95% CI unless otherwise noted. Data are presented as mean±SEM.
Trpv4−/− mice are more susceptible to diet-induced obesity than Trpv4+/+ mice
Trpv4−/− mice weighed significantly more than Trpv4+/+ mice at 10 weeks of age (figure 1A,B). After being fed a high-fat diet, Trpv4−/− mice gained significantly more weight than Trpv4+/+ mice (figure 1A,B). DXA measurements after high-fat feeding revealed that the differences in body mass with genotype and diet were due to body fat, with Trpv4−/− mice gaining significantly more body fat than Trpv4+/+ mice following high-fat feeding (figure 1C). To further examine the obese phenotype of Trpv4−/− mice, histological sections were taken of the inguinal fat pad of 10-week-old normally fed mice and showed that even prior to high-fat feeding, Trpv4−/− mice may possess larger adipocytes than Trpv4+/+ controls (figure 1D).
High-fat fed Trpv4−/− mice have lower cage activity than high-fat fed Trpv4+/+ mice
To further investigate the relationship between Trpv4 deficiency and increased weight gain, spontaneous cage activity was measured after 3 days of habituation. For mice fed a control fat diet, genotype had no effect on dark cycle locomotor activity (p=0.332); yet, when fed a high-fat diet, Trpv4−/− mice were 40% as active as Trpv4+/+ mice (figure 1E).
Trpv4 deficiency increases knee OA following high-fat feeding
A modified Mankin score was tabulated and analysed that combined the score for cartilage structural degeneration and proteoglycan loss as recommended in reference.19 Neither Trpv4−/− nor high-fat feeding alone increased joint degeneration; however, the combination of the two factors increased OA severity (figure 2A,B). Trpv4−/− mice also demonstrated altered chondrocyte histomorphology, with reduced chondrocyte cloning and chondrocyte hypertrophy (figure 2C, see online supplementary Figure S1).
Altered in vitro differentiation of Trpv4−/− progenitor cells
MSCs and ASCs were isolated from 10-week-old Trpv4−/− and Trpv4+/+ mice and expanded to P3. No effect of genotype on expansion rate was observed (figure 3A1). Trpv4−/− MSCs exhibited a reduced adipogenic differentiation potential compared to MSCs isolated from Trpv4+/+ mice, whereas Trpv4−/− ASCs demonstrated a largely increased adipogenic differentiation potential compared to Trpv4+/+ ASCs (figure 3A2,B). Similarly, Trpv4−/− MSCs demonstrated a reduced osteogenic differentiation potential while Trpv4−/− ASCs had an increased osteogenic differentiation potential (figure 3A3,C). No effect of Trpv4 deficiency was observed with MSC chondrogenic differentiation. However, Trpv4−/− ASCs demonstrated a reduced chondrogenic differentiation potential compared to Trpv4+/+ ASCs, as indicated by less GAG/DNA (figure 3D) and Alcian blue staining (figure 3A4).
Our findings indicate that TRPV4 exhibits a chondroprotective role in diet-induced OA. Trpv4−/− mice exhibited an increased susceptibility to high fat diet-induced OA, potentially due in part to an increased susceptibility to diet-induced obesity. MSCs and ASCs from these mice also demonstrated altered differentiation potential, with ASCs from Trpv4−/− mice exhibiting significantly higher adipogenic potential and decreased chondrogenic potential.
Our observations that very high-fat fed Trpv4−/− mice are particularly prone to obesity stands in contrast with the findings by Kusudo et al20 that pan-Trpv4−/− mice are protected from diet-induced obesity. However, substantial differences exist between these two studies, including age at diet initiation, composition, and duration of high-fat feeding. In this study, mice were fed a 60% high-fat diet for 22 weeks, beginning at 10 weeks, compared to a 42% kcal diet beginning at 16 weeks and lasting 12 weeks.20 In addition, although we did not measure food consumption or energy expenditure in our study (no effect of Trpv4 knockout was found by Kusudo et al20), we observed reduced cage activity of high-fat fed Trpv4−/− mice. It is unclear, however, whether this represents a cause and/or a consequence of the additional weight gain of Trpv4−/− mice. Additionally, given the chondroprotective effect of activity in the setting of diet-induced obesity,21 it is even possible that this decrease in activity increased the severity of joint degeneration in the Trpv4−/− mice directly.
While neither high-fat feeding nor Trpv4 deficiency alone increased OA severity at 8 months of age, a combination of these two factors did. This finding is generally consistent with previous work showing that Trpv4−/− mice exhibit significant spontaneous OA changes at 9 months.13 Similarly, high-fat diet feeding alone did not produce a significant increase in OA, but is consistent with previous similarly designed studies. Though this study supports the hypothesis that TRPV4 plays a role in the pathogenesis of obesity-associated OA, further investigation is needed to fully describe the cartilage-specific role of TRPV4 in obesity and OA.
Trpv4−/− mice at 32 weeks of age exhibited a chondrocyte morphology that is distinct from that of Trpv4+/+ mice, with less chondrocyte cloning and hypertrophy observed in the articular cartilage of Trpv4−/− mice. The cause of chondrocyte cloning (or chondrocyte cluster formation) in osteoarthritic cartilage and its influence on cartilage disease progression is unknown, but may signify a proliferative repair response.22 Chondrocyte hypertrophy in articular cartilage also signifies altered metabolic activity by chondrocytes following tissue damage and inflammation.23 ,24 Further studies will be needed to determine if TRPV4 mediates these or other chondrocyte responses to joint insult.
We observed an altered metabolic and osteoarthritic response of Trpv4−/− mice to high-fat feeding. In an attempt to better understand the role of this channel in these mesodermally-derived tissues, we examined whether adult stem cells would also exhibit altered growth or differentiation characteristics in vitro that reflect the tissue characteristics observed in vivo. Isolated MSCs from Trpv4−/− mice exhibited reduced adipogenesis, while ASCs revealed significantly increased adipogenesis. Consistent with these in vitro findings, adipocytes in Trpv4−/− mice appeared larger than those of Trpv4+/+ mice at 10 weeks of age. Determining the role of TRPV4 in adipose tissue function, as is being actively pursued with other TRP channels,25 could yield important insight into both metabolic and inflammatory-associated diseases.
The role of TRPV4 in bone metabolism is also evident in Trpv4−/− mice, which display increased bone volume that may in fact contribute to increased cartilage degeneration directly.13 While the skeletal phenotype of Trpv4−/− mice has been largely attributed to impaired osteoclast function,26 we found that Trpv4−/− MSCs exhibited reduced osteogenesis, while Trpv4−/− ASCs exhibited increased osteogenesis. Future investigations are needed to fully deconstruct the role of TRPV4 in bone development, remodelling and repair.
Though we found no effect of TRPV4 in MSC chondrogenesis, it is plausible that the potent application of growth factors required to induce in vitro chondrogenesis (TGF-β3, BMP-6) may have overpowered the effect of basal TRPV4 signalling in these cells. TRPV4 activation in chondroprogenitors has been shown to enhance Sox9 expression in a Ca2+-dependent manner, one of the main regulators of cartilage-specific expression of matrix molecules such as collagen type II and aggrecan.27 Interestingly, we observed that chondrogenesis was diminished in Trpv4−/− ASCs, possibly due to the altered phenotype and metabolism of the pan-Trpv4−/− adipose tissue, rather than indicating a direct effect of loss of TRPV4 signalling with chondrogenic differentiation. Further studies are necessary to establish the role of TRPV4 in adipose tissue that led to the pro-obesity phenotype in our Trpv4−/− mice.
In conclusion, global loss of Trpv4 increases knee OA severity in response to high-fat feeding in a manner that is associated with increased weight gain. However, the effects of pan-Trpv4 deletion, such as alterations in bone remodelling13 ,26 and energy metabolism, complicates conclusions regarding the in vivo role of various progenitor cells in this model system. There may be other systemic effects of pan-Trpv4 knockout as well, included altered nociception, given the involvement of TRPV4 in joint inflammation and pain.15 ,28 Use of tissue-targeted Cre-lox systems29–31 may help to define the tissue-specific effects of TRPV4 signalling with respect to obesity, joint inflammation, pain and OA. Determining the cartilage-specific role of TRPV4 in the many aetiologies and models of OA, including obesity-induced OA, should provide new insight into molecular mechanisms that link biomechanical and inflammatory factors of OA, hopefully leading to new preventions and treatments for this prevalent disease.
We thank Bridgette Furman and Holly Leddy for help with histological grading and statistical analysis, Brian Diekman and Chia-Lung Wu for assistance with stem cell isolations, and Steven Johnson and Francisco Cordero for their technical support. We would like to thank Drs. William Westel and Ramona Rodriguiz for assistance and advice on the study.
Contributors TMG, WL and FG developed the concept of the study. CJO and TMG designed and carried out the experiments and analysed the data. All authors interpreted the data. CJO wrote the manuscript.
Funding This work was supported by grants from the NIH (AR48182, AR50245, AG15768, AR48852, AG40868, DE018549 and GM08719) and the Arthritis Foundation.
Competing interests None.
Provenance and peer review Not commissioned; externally peer reviewed.