Background Imaging modality for quantitative assessment of inflammation in rheumatoid arthritis (RA) is needed for diagnosis and evaluation of treatment response. Magnetic resonance imaging and 18F-fluorodeoxyglucose positron emission tomography (18F-FDG PET) can be imaging modalities for quantitative assessment of inflammation, however, they have some limitations, such as, long examination time, high cost and the radiation exposure. Recently, fluorescence optical imaging (FOI) has been introduced as a fast and nonionizing imaging modality that offers high sensitivity for the detection of inflammatory arthritis
Objectives This study was performed to evaluate the quantification of joint inflammation after treatment in collagen induced arthritis (CIA) mice using FOI.
Methods To examine the feasibility of FOI to monitor the outcome of a therapeutic intervention, CIA mice were treated with methotrexate (MTX). Before and after treatment with MTX, FOI and 18F-FDG PET analysis were performed. Quantitative analysis of the FOI scans was performed by measuring the fluorescence signal intensity of response to treatment via operator-defined regions of interest. 18F-FDG injected in mice with CIA was analysed by PET
Results ICG enhanced FOI showed a significant decrease in the fluorescence signal of arthritic joints of MTX treated mice at the time point of post-treatment compared with baseline values (p<0.05). The fluorescence signal intensities of response to treatment were significantly associated with the clinical arthritis scores (p<0.05). 18F -FDG uptake was decreased after treatment similarly with FOI and clinical scores.
Conclusions ICG enhanced FOI might be a useful tool for evaluating the quantification of joint inflammation
Irmler IM, et al. In vivo molecular imaging of experimental joint inflammation by combined 18F-FDG positron emission tomography and computed tomography. Arthritis Res Ther 2010;12(6):R203.
Meier R, et al. Indocyanine Green–Enhanced Imaging of Antigen-Induced Arthritis With an Integrated Optical Imaging/Radiography System. Arthritis Rheum 2010;62:2322-7.
Disclosure of Interest None Declared
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