Article Text

PDF
AB0242 Evidence for caveolin-1 (CAV1) as a new susceptibility gene regulating tissue fibrosis in systemic sclerosis
  1. M. Manetti1,2,
  2. Y. Allanore3,4,
  3. M. Saad5,
  4. C. Fatini6,
  5. V. Cohignac4,
  6. S. Guiducci2,
  7. E. Romano2,
  8. P. Airό7,
  9. P. Caramaschi8,
  10. V. Riccieri9,
  11. A. della Rossa10,
  12. R. Caporali11,
  13. G. Cuomo12,
  14. P. Dieudé13,
  15. G. Chiocchia4,
  16. M. Martinez5,
  17. L. Ibba-Manneschi1,
  18. M. Matucci-Cerinic2
  1. 1Anatomy, Histology and Forensic Medicine
  2. 2Biomedicine, Division of Rheumatology, University of Florence, Florence, Italy
  3. 3Rhumatologie A, Hôpital Cochin, APHP, Université Paris Descartes
  4. 4INSERM U1016, Institut Cochin, Paris
  5. 5INSERM UMR1043, CPTP, CHU Purpan, Université Paul Sabatier, Toulouse, France
  6. 6Medical and Surgical Critical Care, University of Florence, Florence
  7. 7Rheumatology and Clinical Immunology, Spedali Civili, Brescia
  8. 8Rheumatology Unit, University of Verona, Verona
  9. 9Division of Rheumatology, Internal Medicine and Medical Specialities, University “Sapienza”, Rome
  10. 10Division of Rheumatology, University of Pisa, Pisa
  11. 11Division of Rheumatology, University of Pavia, IRCCS Policlinico San Matteo Foundation, Pavia
  12. 12Clinical and Experimental Medicine, Rheumatology Unit, Second University of Naples, Naples, Italy
  13. 13Rhumatologie, INSERM U699, Hôpital Bichat Claude-Bernard, Université Paris Diderot, Paris, France

Abstract

Background Caveolin-1 (CAV1) is an inhibitor of tissue fibrosis and has been implicated in the pathogenesis of systemic sclerosis (SSc).

Objectives The aim of our study was to analyse the possible association of CAV1 gene single nucleotide polymorphisms (SNPs) with SSc.

Methods We studied a total population of 3974 individuals of European Caucasian ancestry (1355 patients with SSc and 2619 healthy controls). Genotype data for 23 SNPs (rs17138756, rs4730742, rs2191498, rs10278782, rs1052990, rs6466579, rs4236601, rs926198, rs3779512, rs10256914, rs3807986, rs959173, rs10270569, rs3807989, rs3779514, rs3815412, rs729949, rs6466587, rs9920, rs1049337, rs6959106, rs7795356, and rs2191502) spanning a region of more than 55 kb in the CAV1-CAV2 gene locus at chromosome 7q31.1 were obtained from a genome-wide scan conducted in a French population (564 SSc patients and 1776 controls). Three CAV1 SNPs (rs926198 T/C, rs959173 T/C, rs9920 T/C) displaying the most significant associations with SSc and/or clinical phenotypes were then genotyped in an Italian population (791 SSc patients and 843 controls). CAV1 protein expression in skin biopsies from SSc patients and controls was investigated by immunohistochemistry and Western blotting.

Results In the French population, the CAV1 rs959173 C minor allele showed a significant protective association with susceptibility to SSc (OR 0.71, 95% CI 0.59 to 0.86, padjusted=0.009), and with the subset of patients with limited cutaneous SSc (lcSSc) (OR 0.71, 95% CI 0.56 to 0.89, padjusted=0.018). This association was replicated in the Italian population and strengthened in the combined populations through Cochran-Mantel-Haenszel meta-analysis (SSc: pooled OR 0.81, 95% CI 0.71 to 0.92, p=0.0018; lcSSc: pooled OR 0.80, 95% CI 0.69 to 0.93, p=0.0053). A reduction in CAV1 rs959173 C allele frequency was also observed in patients with diffuse cutaneous SSc compared with healthy controls, although this difference did not reach statistical significance. No significant heterogeneity in ORs was observed between the French and Italian populations according to the Breslow-Day method. As determined by immunohistochemical analyses, individuals who carried the rs959173 C minor allele displayed higher CAV1 protein expression in different cell types, including keratinocytes, dermal endothelial cells and fibroblasts, than those who did not. Western blotting analysis on skin protein lysates confirmed that CAV1 protein expression levels were significantly higher in both SSc patients and controls carrying the CAV1 rs959173 C protective allele than in those carrying the rs959173 TT homozygous genotype (both p<0.01).

Conclusions Our results add CAV1 to the list of SSc susceptibility genes and provide evidence through genotype/phenotype correlation for the direct contribution of this pathway regulating the fibrotic process in SSc pathogenesis.

Disclosure of Interest None Declared

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.