Article Text

AB0229 Senescence associated β-galactosisdase expression in systemic sclerosis skin and fibroblasts
  1. F. Ogawa,
  2. H. Tomita,
  3. Y. Koike,
  4. Y. Kuwatsuka,
  5. A. Utani
  1. Dermatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan


Background Systemic sclerosis (SSc) is connective tissue disease characterized by fibrosis and vascular changes in the skin and internal organs with autoimmune background. However, the mechanism is still unknown. Recently, it is reported that cellular senescence is associated with fibrosis and its amelioration in several diseases. Senescence associated β-galactosidase (SA-β-gal) is one of the histological marker for the cellular senescence.

Objectives To determine association of cellar senescence in SSc skin and fibroblast.

Methods SA-β-gal expression was investigated in skin samples from SSc patients and age- and sex- matched healthy control. SA-β-gal activity was also compared in SSc patients between sclerosis lesion (forearm) and non-sclerotic lesion (upper arm). In addition, SA-β-gal expression was also investigated in fibroblasts from SSc patients and healthy control. Senescent fibroblast established by successive subculture was used for SA-β-gal staining control.

Results Senescence fibroblast showed large-scaled shape and nucleus with SA-β-gal staining. SA-β-gal expression was higher in SSc skin than control. SA-β-gal activity was observed mainly in upper dermis. Furthermore, SA-β-gal expression was observed in sclerotic skin lesion of SSc patients, however, its expression was not detected in non-sclerotic lesion of SSc patients. Furthermore, fibroblast from SSc patients showed higher SA-β-gal expression than control fibroblast.

Conclusions These results suggest that cellular senescence in SSc fibroblast may contribute to disease development in patients with SSc

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Disclosure of Interest None Declared

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