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AB0227 PSGL-1 deficiency develops systemic sclerosis in mice
  1. A. Urzainqui1,
  2. A. Perez-Frias1,
  3. R. Gonzalez-Tajuelo1,
  4. N. Nuñez-Andrade1,
  5. R. Tejedor1,
  6. C. Muñoz-Calleja1,
  7. S. Castañeda2,
  8. E. Vicente2,
  9. C. Gamallo3,
  10. J. Fraga3,
  11. A. García-Diez4
  1. 1Inmunologia
  2. 2Reumatologia, Instituto de Investigacion Sanitaria-Hospital De La Princesa
  3. 3Anatomia Patologica, Hospital De La Princesa
  4. 4Dermatologia, Instituto de Investigacion Sanitaria-Hospital De La Princesa, Madrid, Spain

Abstract

Background Previous studies in our lab have established that PSGL-1 signaling in human monocyte-derived dendritic (DC) cells induces a tolerogenic program that enables them to promote the differentiation of naïve T cells to T regulatory cells (Treg). We have also found that PSGL-1 modulates the immune inflammatory response in the murine enteric lamina propria, indicating that this adhesion receptor plays a role in the control of peripheral tolerance, acting as a tolerogenic receptor.

Objectives To study the spontaneous disease developed in PSGL-1 deficient mice and and analyze its evolution with aging

Methods Inmunological study of skin infiltrate, Serological analysis and histological studies of skin, lungs and kidney of C57/Bl6 WT and PSGL-1 deficient mice

Results we have observed that 30% of PSGL-1 deficient mice at the age of 3 months present skin ulcers in their back that get more severe as the animals get older. The immunological studies show an inflammatory environment in the skin of the PSGL-1 KO mice, with activated and pro-inflammatory DC and macrophage populations, increased populations of granulocytes and B lymphocytes and increased Teff/Treg ratio. In addition, these animals cannot maintain the back legs extended, suggesting muscle weakness, and do not gain weight after the age of 1 year, probably due to intestinal absorption problems. The serum analysis shows the presence of connective tissue disease-related autoantibodies that accumulate as the mice get old. By histological studies we have found that the skin of PSGL-1 deficient mice is altered, with loss of the fat layer and thickening of the dermis by collagen accumulation, a major criterion supporting a diagnosis of Scleroderma. We have also found that the old PSGL-1 deficient mice have infiltrating leukocytes in the lungs and in the kidneys, indicating systemic autoimmune disease.

Conclusions All these data indicate that PSGL-1 has an important role in maintaining the homeostasis of the immune system and controlling the development of systemic sclerosis

Disclosure of Interest None Declared

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