Article Text

AB0067 Total glucosides of paeony attenuated maturation of dendritic cells via blocking TLR4&5 signaling pathway leading to inhibit inflammation in vivo
  1. N.L. Li1,1,
  2. J.L. Lin,
  3. Z.Z. Zhou,
  4. R. Huo,
  5. N. Li
  1. Shanghai Institute of Immunology, Shanghai, China


Background Total glucosides of paeony (TGP), as active compounds extracted from the roots of Paeonia lactiflora Pall, have been recognized as an effective agent on improvement of inflammation in rheumatoid arthritis (RA) patients for more than 10 years. Nevertheless, whether TGP play roles on DCs maturation remains unknown.

Objectives This study aims to investigate the effect and mechanisms of Total glucosides of paeony (TGP) in DC maturation and its effect on inflammation alteration in vivo.

Methods TGP inhibiting DC maturation and antigen presentation was studied in Ovalbumin (OVA)-challenged inflammatory model. TGP preventing onset and clinical inflammation was examined in collagen induced arthritis (CIA) mice model. Expression of cytokines was measured by ELISA, real-time PCR and flow cytometry. Signaling and nuclear transcriptional factor profiles were detected by real-time PCR, western blotting or confocal microscopy.

Results TGP attenuated maturation of DCs selectively via blocking activation TLR4&5/MyD88/NF-kB&AP1 signaling pathway. These in turn arrested Th1 and Th17 activation and differentiation followed by inflammation reduction in OVA-challenged mice in vivo. In TGP treated CIA model, mDCs was reduced companied by down-regulated immune response against collage II and frequency of Th1/Th17, leading to delayed onset and decreased inflammatory clinical score.

Conclusions TGP arrest DCs maturation leading to reduce Th1/Th17 activation in vivo, adding a novel mechanism and therapeutic target of TGP for autoimmune diseases treatment, such as RA. Moreover, it seemed to us that TGP might be advantages in treatment RA in early-onset or an earlier resort to arresting relapse diseases trigged by re-exposure to various activators.

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Disclosure of Interest None Declared

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