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AB0057 Influence of PVA coated nanoparticles on survival and functionality of human immune cells
  1. C. Strehl1,2,
  2. T. Gaber1,2,3,
  3. M. Wagegg1,
  4. M. Jakstadt1,2,3,
  5. M. Hahne1,2,4,
  6. S. Schellmann1,2,
  7. B. Esche-Szostak2,
  8. G. Coullerez5,
  9. H. Hoffmann5,
  10. G.-R. Burmester2,
  11. F. Buttgereit2
  1. 1German Rheumatism Research Centre (DRFZ)
  2. 2Department of Rheumatology and Clinical Immunology
  3. 3Berlin-Brandenburg Center for Regenerative Therapies (BCRT)
  4. 4Berlin-Brandenburg School for Regenerative Therapies (BSRT), Charité University Medicine, Berlin, Germany
  5. 5Institute of Materials Powder Technology Laboratory, Έcole polytechnique fédérale de Lausanne EPFL, Lausanne, Switzerland

Abstract

Background Nanotechnology has developed into a key technology of the 21st century. Over the recent years, the number of nanotechnical products has received an enormous boost. More and more efforts are being done to use this technology in human medicine for diagnostic and therapeutic purposes. Therefore, crucial questions concern the safety aspects. The focus of our work here was to identify possible effects of nanoparticles on human immune cell function.

Objectives We analysed clinical relevant interactions between PVA coated nanoparticles (spions) and human immune cells.

Methods 100μl of whole blood obtained from patients with rheumatoid arthritis (RA) or healthy donors were incubated with 100μl serum free RPMI 1640. Functionalised spions were added at varying concentrations, and cells were incubated for 24h. After lysis of erythrocytes, cells were stained for apoptosis and necrosis using Annexin V and 7AAD, respectively. Samples were analysed by flow cytometry. As a second approach, PBMCs were isolated from blood samples of healthy donors and RA patients, and CD4 positive T cells were separated via MACS-Sort. T cells were incubated with/without PHA and/or with/without PVA spions at different concentrations. Activation (CD25 expression) of cells was analysed by flow cytometry. Functionality was determined via proliferation measurements of CFSE (carboxyfluorescein diacetatesuccinimidyl ester) labeled T cells after 72h under normoxic (5% CO2 and 18% O2) or hypoxic (5% CO2 and <1% O2) conditions by flow cytometry.

Results Altogether, blood samples from 18 healthy donors and 19 patients suffering from RA were analysed for induction of apoptosis and necrosis in different cell types. The results on cell survival did not demonstrate any short-term general toxicity of PVA spions at concentrations less than 1000μg/ml on the several different blood cell subsets examined. Furthermore, T cells were isolated from 14 healthy donors and 9 RA patients for functional analysis. There is no influence of PVA spions on T cell activation and proliferation at concentrations less than 1000μg/ml.

Conclusions PVA coated nanoparticles at concentrations up to 1000μg/ml (i) do not increase the frequencies of apoptotic or necrotic human immune and (ii) do not impair crucial functional activities of human T cells such as activation and proliferation.

Disclosure of Interest None Declared

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