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AB0020 Vascular endothelial growth factor gene polymorphism in patients with rheumatoid arthritis
  1. M. Korolev,
  2. V. Konenkov,
  3. V. Prokofyev,
  4. A. Shevchenko,
  5. E. Zonova
  1. Rheumatology, Institute of Clinical and Experimental Lympology, Novosibirsk, Russian Federation

Abstract

Background Vascular endothelial growth factor (VEGF) have recently reported to be expressed in human rheumatoid arthritis (RA), suggesting that VEGF could be implicated in the pathogenesis of this disease.

Objectives The aim of this study was to investigate the frequency of occurrence of certain alleles and genotypes of VEGF gene among Caucasoid RA patients living for a long time in the Siberian region of Russia, in comparison with a group of healthy individuals.

Methods A total of 509 Russian individuals were enrolled in this study, including 135 patients with RA satisfied (ACR, 1987) criteria and 374 healthy controls. Eight different polymorphisms of the VEGF gene and pro- and anti-inflammatory cytokines genes were analyzed using method of restriction analysis of amplified products (PCR-RLFP) with matching restrictases (VEGF -2578 A/C – BlgII, TNFA C-863A - Bst BAI, TNFA G-308A - Bsp19 I, TNFA G-238A - Msp I, IL-1B T-31C - Alu I, IL-4 C-590T - Bme 18 I, IL-6 G-174C - SfaN I, IL-10 C-592A - RsaI).

Results The resulting study data suggest that the combination of genotypes of VEGF gene polymorphism in the promoter region at -2578 A/C genotypes with genes IL1B -311C/T, IL4 -590 C/T, IL10 -592 A/C, IL6 -174 G/C and TNFA gene may be genetic factors that allow population to discriminate against women in Russian Caucasoid descent into groups with high risk of developing RA and with a certain level of constitutive resistance to its development.

Conclusions Our conclusion indicates the need for a comprehensive study of the role of the growth factors family with proangiogenic and proinflammatory activity in the pathogenesis of RA.

Disclosure of Interest None Declared

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