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SAT0180 B7-H4 expression of renal tubular epithelial cells in patients with lupus nephritis
  1. X. Li,
  2. D. Yu,
  3. X. Wang,
  4. X. Li,
  5. G. Wang
  1. Department of Rheumatology and Immunology, Anhui Medical University Affiliated Provincial Hos, Hefei, China

Abstract

Background B7-H4 is a newly identified B7 family negative co-stimulate molecule, the expression and function of B7-H4, in the pathogenesis of lupus nephritis (LN) is still unclear.

Objectives To investigate the expression of B7-H4 in patients with LN and the function of renal tubular epithelial cells (TECs)-associated B7-H4 in the regulation of T cell activation in vitro.

Methods 90 LN patients, 3 acute kidney injury patients and 20 healthy donors were referred. Disease activity was assessed by SLEDAI scores. The expression of B7-H4 on kidney biopsies from patients with LN and acute kidney injury was measured using immunohistochemistry. In vitro, B7-H4 antigen on cultured HK-2 cells with or without stimulation by inflammatory factors was detected by flow cytometry. After co-cultured with HK-2 and purified CD4+T cells labeled with CFSE for 72 hours, T cell proliferation was detected by flow cytometry. The soluble B7-H4 in sera of LN patients and healthy donors were analyzed using ELISA.

Results B7-H4 antigen expressed on tubular epithelium, the percentage of B7-H4 positive expression renal tubules from LN patients (45±16) % was lower than acute kidney injury patients (86±11) % (P<0.05). In vitro, the expression of B7-H4 on HK-2 cells was elevated upon stimulation by inflammatory factors, mixed lymphocyte reactions revealed that HK-2-related B7-H4 inhibits proliferation of co-cultured T cells. The soluble B7-H4 in serum of LN patients (61.45±29.38ng/ml) was not significantly lower than healthy controls (70.57±27.24ng/ml), but stronger association of serum soluble B7-H4 with serum creatinine levels was observed in LN patients (r=0.353, p=0.005). In addition, the mean concentration of soluble B7-H4 level in high activity group (74.40±31.95 ng/ml) was significantly higher than those in moderate group (51.89±21.79 ng/ml) (p=0.017)

Conclusions B7-H4 molecules may play a role in the progress of LN, a clear understanding of its functional roles may further elucidate the pathogenesis of this disease.

Disclosure of Interest None Declared

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