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Scientific Abstracts
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Cytokines and inflammatory mediators
FRI0027 Pro-resolving lipid mediators are present in the joints of osteoarthritis patients
  1. M. Giera1,
  2. C. Serhan2,
  3. M. Kloppenburg3,
  4. O. Mayboroda1,
  5. A. Deelder1,
  6. R. Toes3,
  7. A. Ioan-Facsinay3
  1. 1Parasitology, Leiden University Medical Center, Leiden, Netherlands
  2. 2Anesthesiology, Perioperative and Pain Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, United States
  3. 3Rheumatology, Leiden University Medical Center, Leiden, Netherlands

Abstract

Background Pro-resolving lipid mediators (LM), such as resolvins, protectins and maresins, are powerful molecules that have a critical role in limiting inflammation and promoting tissue regeneration. Biochemically they are derived from the enzymatic oxygenation of poly-unsaturated fatty acids by lipoxygenases (LOX). Some LOX involved in the generation of pro-resolving LM have been suggested to play a role in the pathophysiology of osteoarthritis (OA) in humans (1,2). However, it is currently unclear whether pro-resolving pathways are activated in the osteoarthritic joint, as the presence of pro-resolving LM or their precursors was not yet investigated.

Objectives We aimed at investigating the presence and abundance of pro-resolving LM and their precursors in synovial fluid (SF) of OA patients compared to Rheumatoid Arthritis (RA) patients.

Methods SF samples from OA (n=8) and RA (n=9) patients visiting the outpatient clinic of the department of Rheumatology were collected. A targeted LC-MS (MS) lipidomics platform based on a QTrap mass spectrometer for the highly sensitive determination of several lipid mediators was used.

Results The presence of LM could be proven in 4 of the OA and 5 of the RA patient samples. The pro-resolving LM resolvin D5 and maresin 1 were readily detectable and were accompanied by the presence of pro-inflammatory mediators such as leukotriene B4 and anti-inflammatory LM, such as lipoxin A4 and B4. Notably, the pro-inflammatory prostaglandins E2 and D2 could be detected in only 1 OA sample, while they were present in all 5 RA samples. Hydroxy derivatives of polyunsaturated fatty acids (HETE, HEPE and HDHA) which are precursors and biomarkers for LM were additionally detected in both patient groups.

Conclusions This study indicates that a previously unrecognized pathway is likely activated in the knee joint of OA patients, as evidentiated by the presence of pro-resolving LM and their precursors in SF of these patients.

  1. Arthritis Research & Therapy 2009, 11:R44

  2. Arthritis Research & Therapy 2008, 10:R85

Disclosure of Interest None Declared

https://doi.org/10.1136/annrheumdis-2012-eular.2484

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