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OP0262 Is enthesitis the primary immunopathological lesion in HLA-B27-associated experimental and human spondyloarthritis?
  1. J. Paramarta1,
  2. C. van der Leij2,
  3. I. Gofita1,
  4. N. Yeremenko1,
  5. M. van de Sande1,
  6. M. De Hair1,
  7. P.P. Tak1,
  8. J. Taurog3,
  9. M. Maas2,
  10. D. Baeten1,
  11. L. van Duivenvoorde1
  1. 1Division of Clinical Immunology and Rheumatology
  2. 2Department of Radiology, Academic Medical Center/University of Amsterdam, Amsterdam, Netherlands
  3. 3Rheumatic Diseases Division, Department of Internal Medicine, UT Southwestern Medical Center, Dallas, TX, United States

Abstract

Background A magnetic resonance imaging (MRI) study of knee arthritis demonstrated that enthesitis was more frequently observed in established spondyloarthritis (SpA) than rheumatoid arthritis (RA), leading to the hypothesis that enthesitis rather than synovitis is the primary lesion in SpA.1,2Although this hypothesis provides a useful conceptual framework to investigate the pathophysiology of SpA, very few studies have confirmed these findings.

Objectives The aim of this study was to assess the presence of enthesitis in the HLA-B27/Huβ2m tg rats3 and in early untreated human SpA.

Methods Histological samples were obtained at different stages of spontaneous tail spondylitis (n=10) and peripheral arthritis (n=9) in HLA-B27/Huβ2m tg rats. Paraffin sections were stained with H&E or toluidine blue and evaluated by two independent observers. Synovial biopsies and MRI were performed in 41 patients with early (less than 1 year) untreated knee or ankle arthritis, who were diagnosed with SpA (n=13), RA (n=20) or crystal arthropathy (n=8) at follow-up. MRI evaluation of enthesitis, synovitis and osteitis and immunohistochemical characterization of synovitis (CD3, CD22, CD68, CD163 and von Willebrand Factor) were performed by two observers blinded to diagnosis.

Results Experimental HLA-B27-related spondylitis and peripheral arthritis were characterized by a progressive inflammatory infiltration of the stromal tissues with mono- and polymorphonuclear cells. In moderate to severe inflammation, this was associated with progressive destruction of bone and cartilage, inflammatory infiltration of the underlying bone, and enchondral bone formation. The entheseal sites of the axial and peripheral joints were not affected as they did neither display signs of inflammation nor osteoproliferation.

In human early untreated arthritis immunohistochemical analysis revealed a similar degree of synovitis in SpA and RA, with a significant increase of sublining CD163 in SpA. On MRI, the synovitis score was even higher in SpA (1.3; SD 0.7) than RA (0.7; SD 0.6). In contrast, there were no differences in prevalence of enthesitis as assessed by peri-entheseal focal tissue edema (67% vs. 75%), peri-entheseal focal bone marrow edema (0% vs. 10%) and entheseal enhancement (46% vs. 47%). Also the number of peri-entheseal lesions did not differ between SpA and RA.

Conclusions This combined histological and imaging evaluation of experimental and early human disease show that enthesitis is not a specific feature of SpA, which challenges the concept that enthesitis would be the primary immunopathological lesion.

  1. McGonagle D, Gibbon W, Emery P. Classification of inflammatory arthritis by enthesitis. Lancet. 1998; 352(9134):1137-40.

  2. McGonagle D, Gibbon W, O’Connor P, Green M, Pease C, Emery P. Characteristic magnetic resonance imaging entheseal changes of knee synovitis in spondylarthropathy. 1998; 41(4):694-700.

  3. Tran TM, Dorris ML, Satumtira N, Richardson JA, Hammer RE, Shang J, Taurog JD. Additional human beta2-microglobulin curbs HLA-B27 misfolding and promotes arthritis and spondylitis without colitis in male HLA-B27-transgenic rats. Arthritis Rheum. 2006; 54(4):1317-27.

Disclosure of Interest None Declared

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