Article Text

PDF

IL-32γ and streptococcus pyogenes cell wall fragments synergise for IL-1-dependent destructive arthritis via upregulation of TLR-2 and NOD2
  1. B Heinhuis1,
  2. M I Koenders1,
  3. F A van de Loo1,
  4. P L E M van Lent1,
  5. S-H Kim3,
  6. C A Dinarello3,
  7. L A B Joosten2,
  8. W B van den Berg1
  1. 1Rheumatology Research and Advanced Therapeutics, Department of Rheumatology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
  2. 2Department of Medicine, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
  3. 3Division of Infectious Diseases, University of Colorado, Denver, Colorado, USA

Statistics from Altmetric.com

Purpose

Interleukin 32 (IL-32) might play an important role in the pathogenesis of RA by inducing cytokines and chemokines. IL-32 is highly expressed in RA synovial tissue and strongly correlates with synovial inflammation, tumour necrosis factor (TNF)α and IL-1 expression. IL-32 alone is not that potent, but it appears to enhance sensitivity of synovial cells to proinflammatory stimuli. Here, we investigated potential synergistic effects of IL-32γ with Streptococcus pyogenes cell wall fragments, which contain predominantly TLR2/NOD2 ligands, in human fibroblast-like synoviocytes (FLS). In addition, we explored the synergistic arthritogenicity of IL-32γ and SCW fragments in wildtype and IL-1 deficient mice.

Method

FLS from 10 arthritis patients were isolated and transduced with an adenoviral vector encoding for IL-32γ (AdIL-32γ) followed by SCW stimulation. To investigate in vivo synergy, we injected AdIL-32γ virus together with SCW fragments intra-articularly into knee joints of wildtype and IL-1 deficient mice.

Results

In human FLS synergistic upregulation of the proinflammatory cytokines IL-1β, IL-6 and TNFα was found and similar observation was made for the chemokines CCL2, CCL20 and CXCL8. Of great importance, we also identified synergistic upregulation of matrix degrading enzymes, including MMP1 and MMP3, linking the IL-32 synergy to erosive processes. Part of the synergy may be related to the marked increase of the pattern recognition receptors TLR2 and NOD2. In wildtype mice we observed a prolonged and severe arthritis injected with AdIL-32γ/SCW compared to AdControl/SCW. Furthermore, histological analysis showed apart from enhanced numbers of inflammatory cells, severe cartilage proteoglycan depletion, and irreversible chondrocyte death in mice exposed to IL-32γ and TLR2/NOD2 ligands. Remarkably, IL-1 deficient mice injected with AdIL-32γ/SCW were completely protected against the destructive arthritis observed in wildtype mice.

Conclusions

The observed synergistic effect between IL-32γ and TLR2/NOD2 ligands identifies an important amplifying pathway in the development of destructive arthritis which is IL-1 dependent via the upregulation of TLR-2 and NOD2 receptors. Targeting of IL-32γ may provide a novel therapy to prevent destructive arthritis.

View Abstract

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.