Tumour necrosis factor alpha blockade impairs dendritic cell survival and function in rheumatoid arthritis
- Institute of Cellular Medicine, Musculoskeletal Research Group, Newcastle University, Newcastle-upon-Tyne, UK
- Correspondence to Professor J D Isaacs, Musculoskeletal Research Group, 4th floor Catherine Cookson Building, Newcastle University, Newcastle- upon-Tyne NE2 4HH, UK;
- Accepted 25 August 2009
- Published Online First 22 September 2009
Objectives Tumour necrosis factor alpha (TNFα) blockade is an effective therapy for rheumatoid arthritis (RA). The immunomodulatory effects of TNFα antagonists are thought to contribute to their therapeutic action. This study investigated whether anti-TNFα therapeutics exerted their immunoregulatory effects through modulation of dendritic cell (DC) function.
Methods Two complementary approaches were taken: in the first ‘in vitro’ approach monocyte-derived DC from healthy donors were matured with lipopolysaccharide and treated with TNFα antagonists in vitro for 48 h. In the second ‘ex vivo’ approach monocyte-derived DC were generated from RA patients before and 8–12 weeks into anti-TNFα treatment. DC were analysed for survival, phenotype, cytokine production and T-cell stimulatory capacity.
Results TNFα blockade during DC maturation in vitro induced approximately 40% of DC to undergo apoptosis. Importantly, the surviving DC displayed a semimature phenotype with reduced levels of HLA-DR, CD80, CD83, CD86 and CCR7, and their production of IL-10 was enhanced compared with DC matured without TNFα antagonists. Furthermore, anti-TNFα-treated DC were poor stimulators of T-cell proliferation and polarised T-cell development towards a higher IL-10/lower IFNγ cytokine profile. Similarly, DC derived from RA patients after anti-TNFα treatment showed impaired upregulation of CD80 and CD86 upon lipopolysaccharide activation and displayed poor T-cell stimulatory activity.
Conclusions The data show that TNFα blockade has profound effects on DC function with downstream, potentially immunoregulatory, effects on T cells. These data provide an interesting new insight into the potential mechanism by which anti-TNFα drugs contribute to the restoration of immunoregulation in RA patients.
JDI and CMUH contributed equally.
Funding This work was supported by the Oliver Bird programme from the Nuffield Foundation, the Arthritis Research Campaign (grant no 16361) and the Society of Japanese Pharmacopoeia for Dr Kazuo Suzuki's project of the Ministry of Health, Labour and Welfare in Japan.
Competing interests None.
Ethics approval Peripheral blood samples were obtained with informed consent and following approval by the Newcastle and North Tyneside Research Ethics Committee 2.
Provenance and peer review Not commissioned; externally peer reviewed.