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Comparison of real-time PCR and nested PCR for the detection of Y chromosome sequences in the peripheral blood mononuclear cells of patients with systemic sclerosis
  1. M Mosca1,
  2. T Giuliano1,
  3. M Curcio2,
  4. M Doveri1,
  5. F De Feo1,
  6. C Tani1,
  7. L Bazzichi1,
  8. S Bombardieri1
  1. 1
    Rheumatology Unit, Department of Internal Medicine, University of Pisa, Italy
  2. 2
    Immunohematology and Transfusional Medicine Unit, Azienda Ospedaliera Universitaria Pisana, Pisa, Italy
  1. M Mosca, Rheumatology Unit, Department of Internal Medicine, University of Pisa, Via Roma 67, 56126 Pisa, Italy; marta.mosca{at}int.med.unipi.it

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Real-time PCR (RT-PCR) is a sensitive technique that has been recently applied to the determination and quantification of fetomaternal microchimerism.13 In a previous manuscript, we detected the presence of Y chromosome sequences in female patients with systemic lupus erythaematosus (SLE) using nested PCR.4 In the present study we evaluated the presence of Y chromosome sequences in the peripheral blood mononuclear cells (PBMCs) of female patients with systemic sclerosis (SSc) using nested and RT-PCR, correlating the results with the clinical and serological manifestations of the patients.

Genomic DNA was isolated from …

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