Amelioration of human lupus-like phenotypes in MRL/lpr mice by overexpression of interleukin 27 receptor α (WSX-1)
- N Sugiyama1,
- H Nakashima1,2,
- T Yoshimura3,4,
- A Sadanaga1,
- S Shimizu1,
- K Masutani5,
- T Igawa1,
- M Akahoshi1,
- K Miyake1,2,
- A Takeda3,4,
- A Yoshimura4,
- S Hamano6,
- H Yoshida7
- 1Department of Medicine and Biosystemic Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
- 2Division of Nephrology and Rheumatology, Department of Internal Medicine, Fukuoka University, School of Medicine, Fukuoka, Japan
- 3Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
- 4Division of Molecular and Cellular Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
- 5Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
- 6Department of Parasitology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
- 7Department of Biomolecular Sciences, Faculty of Medicine, Saga University, Saga, Japan
- H Nakashima, Division of Nephrology and Rheumatology, Department of Internal Medicine, Fukuoka University, School of Medicine, 7–45–1 Nanakuma, Jonann-ku, Fukuoka 814–0180, Japan; and H Yoshida, Department of Biomolecular Sciences, Faculty of Medicine, Saga University, Saga, 849–8501, Japan;
- Accepted 30 November 2007
- Published Online First 18 December 2007
Objective: In the present work, we investigate the role of interleukin (IL)27/IL27 receptor α (Rα) (WSX-1) in the development of autoimmune disorders in the MRL/lpr mouse, which is considered as an experimental model of systemic lupus erythaematosus (SLE) in humans.
Methods: We generated two strains of WSX-1 transgenic mice in the MRL/lpr background with different expression levels of WSX-1, and investigated the effect of WSX-1 overexpression on survival, glomerulonephritis and immunological properties.
Results: In comparison with wild type (WT) MRL/lpr and transgenic (Tg) low (TgL) mice, Tg high (TgH) mice exhibited a prolonged lifespan and no apparent development of autoimmune nephritis. Production of anti-dsDNA antibody and total IgG and IgG2a were significantly lower in TgH mice than those of TgL and WT mice. The expressed amounts of interferon (IFN)γ and IL4 mRNA by CD4+ T cells from Tg mice decreased in a dose-dependent fashion. CD4+ splenic lymphocytes in TgH mice were more subject to the IL27-mediated suppression of cytokine production. In vitro stimulation of CD4+ T cells by IL27 resulted in over phosphorylation of STAT3 in TgH cells than in WT cells.
Conclusion: WSX-1 overexpression in the MRL/lpr background rendered the autoimmune prone mice protected from the development of autoimmune diseases. Our results suggest that IL27 signalling may be a therapeutic target against autoimmune diseases, including human SLE.
Funding: This study was supported in part by grants from the Ministry of Education, Science, Technology, Sports and Culture of Japan (to AY, SH and HY), from Japan Research Foundation for Clinical Pharmacology (to HY), from the Sumitomo Foundation, Grant for Basic Science Research Projects (to HY), from the Naito Foundation (to HY), and from the Takeda Science Foundation (to HY). This work was also supported by the president’s expenditure (research project expenditure) of Saga University (to HY).
Competing interests: None declared.