Intrarenal cytokine gene expression in lupus nephritis
- Rebecca Wing-Yan Chan1,
- Fernand Mac-Moune Lai2,
- Edmund Kwok-Ming Li1,
- Lai-Shan Tam1,
- Kai-Ming Chow1,
- Ka-Bik Lai1,
- Philip Kam-Tao Li1,
- Cheuk-Chun Szeto1
- 1Department of Medicine & Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, China
- 2Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, China
- Correspondence to:
Dr C C Szeto
Department of Medicine & Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China;
- Accepted 18 February 2007
- Published Online First 26 February 2007
Background: Lupus nephritis is characterised by intrarenal inflammation and lymphocyte activation.
Aim: To examine the profile of cytokine gene expression in glomerulus and tubulointerstitium in patients with lupus nephritis.
Methods: 36 consecutive patients with systemic lupus erythematosus having active renal disease were recruited, and they were required to undergo kidney biopsy. Glomerular and tubulointestitial cytokine expression of interleukin (IL)2, 4, 10, 12, 18, interferon γ (IFN)γ, T-bet (the Th1 transcription factor), GATA-3 (the Th2 transcription factor), transforming growth factorβ and monocyte chemoattractant protein (MCP)1 were studied by laser microdissection of the renal biopsy specimen, followed by real-time quantitative PCR.
Results: There were 13 patients with World Health Organization class III nephritis, 14 patients with class IV nephritis and 9 patients with class V nephritis. There was a significant correlation between serum C3, C4 and anti-double strand DNA antibody level with glomerular expression of T-bet, IFNγ and IL2. There was a significant correlation between histological activity index and glomerular expression of IL12, IL18, IL10 and MCP1. In addition, the degree of glomerular leucocyte infiltration significantly correlated with glomerular expression of IFNγ, IL10, IL12 and IL18. By contrast, histological chronicity index correlated with the tubulointerstitial expression of IL2, MCP1 and GATA-3.
Conclusions: Intraglomerular expression of certain target genes correlate with the severity of systemic as well as histological activity, whereas the tubulointerstitial expression of other target genes correlate with the degree of chronic kidney scarring. This result may shed light on the immunopathogenesis of lupus nephritis.
- AI, activity index
- CI, chronicity index
- ds, double strand
- IFN, interferon
- IL, interleukin
- MCP, monocyte chemoattractant protein
- mRNA, messenger RNA
- RT-QPCR, real-time quantitative PCR
- rRNA, ribosomal RNA
- SLE, systemic lupus erythematosus
- SLEDAI, Systemic Lupus Erythematosus Disease Activity Index
- WHO, World Health Organization
Published Online First 26 February 2007
Competing interests: None.