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Smoking may affect the disease course in SLE and patients should be counselled to stop
In this issue of the Annals, Freemer et al report an association between smoking and dsDNA autoantibody production in systemic lupus erythematosus (SLE).1 The authors note that exposure to tobacco smoke has previously been associated with several autoimmune diseases, including rheumatoid arthritis (RA) and SLE. In RA, cigarette smoking has been associated with rheumatoid factor (RF) positive but not RF negative disease when these two groups of subjects were evaluated separately.2–4 Likewise, smoking has been associated with anti-cyclic citrullinated antibody (anti-CCP) positive but not anti-CCP negative RA.5 In affected subjects, exposure to tobacco has also been associated with several measures of disease severity such as the presence of radiographic erosions, nodules, pulmonary disease, RF, and anti-CCP antibodies.5–8 A cumulative dose of exposure has been associated with a higher incidence and prevalence of RA as well as RF and anti-CCP positivity5,7–9; in studies evaluating intensity and duration separately, it appears that duration is most important.3,10 Smoking has been identified as a risk factor for seropositive RA incidence,2,4 and a few studies have even identified an association between cigarette smoking and RF positivity in subjects without RA,11–13 supporting a role for this exposure very early during the development of clinical disease. In this regard, it has been proposed that exposure to tobacco may trigger RF production, thus contributing to the clinical onset of RA.14
SLE DEVELOPMENT AND CIGARETTE SMOKING
While tobacco exposure has been causally linked to RA, the relationship between the development of SLE and cigarette smoking is less clear. A recent meta-analysis identified a modest association between the development of SLE and current, but not former smoking (odds ratio (OR) = 1.50, 95% confidence interval (CI) 1.09 to 2.08).15 One limitation of that analysis is that the majority of the studies included did not have statistically significant associations and one outlying study had a high point estimate for the relationship (OR = 6.7) in a predominately Hispanic population.16 Furthermore, in two studies examining the relationship between smoking and incident SLE, statistically significant associations were not identified.17,18 Nevertheless, the previous identification in affected subjects of associations between smoking and severity of SLE may indicate that a relationship exists between smoking and SLE once clinical disease is established. For example, Ward and Studenski identified an association between SLE and progression of lupus nephritis to end stage renal disease.19 Additionally, smokers were found to have significantly higher Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) scores and a higher odds of thrombotic events.20,21
As in the analysis of RA related autoantibodies, studies examining the association between tobacco exposure and presence of autoantibodies in clinically unaffected subjects are limited. One previous study found an increased prevalence of antinuclear antibodies (ANA) in smokers from a general population cohort,12 while a recent small study found a negative correlation between smoking and IgG anti-DNA antibodies in subjects with SLE.22
With this background, the investigations undertaken by Freemer et al were warranted and their findings are a useful addition to this area of investigation. They found that current versus never smoking is associated with the presence of autoantibodies to double stranded DNA (dsDNA) (OR = 4.0, 95% CI 1.6 to 10.4) as was current versus former smoking (OR = 3.0, 95% CI 1.3 to 7.1). The OR for the association of ever smoking with dsDNA positivity was 1.5, and they found no relationship between dsDNA status and the duration of smoking in former smokers. These results are consistent with the meta-analysis of Costenbader et al showing that current smoking rather than former smoking is most important in risk assessment for SLE development.15
“Current smoking rather than former smoking is a greater risk factor for development of SLE”
Potential pitfalls of this study are relatively few and are mainly due to the inherent limitations of a case-control study. For example, smoking classification at the time of dsDNA testing may not be accurate given that it was collected by chart review. In the ideal setting, serum or urinary cotinine levels would provide a more accurate estimate of subjects’ smoking status. Additionally, the magnitude of the association between smoking and dsDNA may have been attenuated by drug treatment for SLE, given that dsDNA is a marker of disease activity in SLE. In addition, the sample size limited the authors’ ability to assess the relationship in different racial and ethnic groups. Such an assessment might be useful given that the point estimate for the association between tobacco and dsDNA was quite high (OR = 6.7) in a predominately Hispanic cohort.16 Finally, as with any study evaluating SLE development and severity, as well as cigarette exposure, adjustment for socioeconomic status is also important.
POSSIBLE MECHANISMS FOR ASSOCIATION BETWEEN CIGARETTE SMOKING, SLE, AND RA
What is the mechanism for the identified associations between cigarette smoking and autoimmune connective tissue diseases? The molecular mechanisms causing the association between smoking, RA, and severity in RA have not been determined. However, several plausible hypotheses have been presented. Genetic susceptibility certainly may have a role, with the recent determination that a null polymorphism in the glutathione S-transferase (GST) M1 locus affects the association between RF and smoking in subjects with RA.23 The GST enzymes are believed to play an important part in detoxifying reactive oxygen species and, therefore, might influence the ability to detoxify chemicals in cigarette smoke. Additionally, another gene-environment interaction has been proposed, in which smoking might cause a modification of potential autoantigens being recognised by T cells that are restricted by major histocompatibility complex (MHC) antigens carrying the shared epitope.4,5 The authors suggest that smoking induces peptide deimination which leads to anti-CCP positive RA in subjects who carry the shared epitope.5 In addition, it has been shown that smoking increases Fas expression in B lymphocytes and CD4+ T lymphocytes.24 Thus, smoking might lead to increased apoptosis, causing exposure to intracellular citrullinated antigens with eventual breakdown of tolerance and induction of RA related autoimmunity, such as anti-CCP and RF production.
For SLE, one very well supported current hypothesis about the nature of pathogenic mechanisms in the disease proposes that ineffective clearance of apoptotic cells due to genetic or acquired deficiencies promotes the loss of self tolerance to nuclear antigens and subsequent B and T cell reactivity.25 Given that cigarette smoke is associated with an influx of short lived apoptosis-prone neutrophils into the lung as well as a decreased ability to clear these cells through phagocytosis by macrophages,26 the generation of anti-dsDNA may be indirect and related to these immunoregulatory effects of tobacco exposure. In this setting, in genetically predisposed subjects with a smoking related decreased ability to clear apoptotic cells, the excess levels of exposed intracellular antigens might lead to a breakdown in tolerance and production of autoantibodies, such as those to dsDNA.24,27
Freemer et al hypothesise that the association between dsDNA and smoking is explained by the formation of DNA adducts with resultant autoantibodies to the damaged DNA. In support of the DNA adduct hypothesis, they cite their observation that the positive association is only present in current smokers and not former smokers. Importantly, antibodies to DNA adducts have been shown to persist after smoking cessation,28 but the authors note that they have a half life of only 9–13 weeks. This hypothesis is plausible, but to date, there is no direct evidence that antibodies against DNA adducts are related to autoantibodies to dsDNA. Furthermore, in the multivariate analysis, the authors found non-significant associations between other ANA and exposure to tobacco, indicating that DNA adducts may not be the mechanism for all autoantibody formation in SLE. In addition, while autoantibodies to dsDNA are directly involved in lupus nephritis, they are not responsible for all the clinical manifestations of SLE and therefore do not fully explain the association between smoking and SLE.
The findings of Freemer et al point to an important area of investigation in the pathogenesis of SLE. Exposure to tobacco has consistently been associated with RA and other autoimmune diseases. Therefore, studies are warranted to further define its relationship with SLE related autoantibodies as well as disease development and clinical course. Investigations aimed at identifying associations with specific organ involvement and disease severity in SLE are indicated. Likewise, studies examining the association between exposure to tobacco and other ANA would help to define further the relationship between smoking and SLE. The concept of a gene-environment interaction is suggested by this study and should be pursued. Certainly, if the association between dsDNA and smoking is due to the formation of DNA adducts, evaluation for GST polymorphism may show that the association between dsDNA and smoking is increased in subjects with SLE who are GSTM1 null.29 Furthermore, studies demonstrating an association between smoking and ANA in subjects without lupus would have the added benefit of demonstrating an association between smoking and autoantibodies to dsDNA which is independent of disease activity. Mechanistic studies in animals would also help to clarify this area.
Finally, smoking is a common habit that is potentially modifiable. Because autoantibodies to dsDNA may affect disease course in SLE, smokers with SLE should be counselled to stop smoking. This is perhaps the most clinically relevant point to be gained from this article.
These studies were supported by K12 RR017707 and the Smyth Professorship in Rheumatology.
Smoking may affect the disease course in SLE and patients should be counselled to stop
Competing Interests: None
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