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The binding of lupus-derived autoantibodies to the C-terminal peptide (83–119) of the major SmD1 autoantigen can be mediated by double-stranded DNA and nucleosomes
  1. J W Dieker1,*,
  2. C C Van Bavel1,*,
  3. G Riemekasten2,
  4. J H Berden1,
  5. J van der Vlag1
  1. 1Nephrology Research Laboratory, Nijmegen Centre for Molecular Life Sciences, Division of Nephrology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
  2. 2Department of Rheumatology and Clinical Immunology, Charité University Hospital, Berlin, Germany
  1. Correspondence to:
    J van der Vlag
    Nephrology Research Laboratory (279), Nijmegen Centre for Molecular Life Sciences, Division of Nephrology, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands; J.vanderVlag{at}NCMLS.RU.NL

Abstract

Objectives: To evaluate the binding of lupus-derived autoantibodies, double-stranded DNA and nucleosomes to the positively charged C-terminal SmD1(residues 83–119) peptide and the full-length SmD protein.

Methods: The binding of lupus-derived monoclonal antibodies, sera from patients with systemic lupus erythematosus, rheumatoid arthritis and systemic sclerosis, dsDNA and nucleosomes to the SmD1(83–119) peptide or the full-length SmD protein was determined using different ELISA methods.

Results: Monoclonal anti-dsDNA antibodies and the serum of patients with systemic lupus erythematosus that are positive for anti-dsDNA antibodies react with the SmD1(83–119) peptide in ELISA. However, DNaseI treatment of the blocking reagents leads to a decreased reactivity. Purified dsDNA and nucleosomes bind to the SmD1 peptide but not to the full-length SmD protein.

Conclusions: The SmD1(83–119) peptide is able to bind dsDNA and nucleosomes, and dsDNA or nucleosomes in applied reagents lead to an apparent reactivity of anti-dsDNA, anti-histone or nucleosome-specific antibodies with the SmD1(83–119) peptide in ELISA.

  • BSA, bovine serum albumin
  • dsDNA, double-stranded DNA
  • SLE, systemic lupus erythematosus
  • SSc, systemic sclerosis

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Footnotes

  • * These two authors contributed equally to the study.

  • Present address: CNRS, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.

  • Competing interests: None.

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